Acquisition of an 800 MHz NMR Spectrometer Console and Probes

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Funding is requested to obtain a Bruker Biospin NMR AVANCE NEO 800 MHz Console and associated components, including BMPC-2 magnet control system, CryoPlatform/5, MASIII pneumatic control unit, TCI-H&F triple-resonance CryoProbe, MAS tri-gamma 1.9 mm solid-state probe, and MAS 3.2 mm solid-state CryoProbe, to be installed on an existing Bruker Biospin 800 MHz NMR magnet system. The present NMR AVANCE I console and TCI CryoProbe/1 were installed 2003 and 2004 and were declared by Bruker Biospin to have reached “End of Service” (EOS) in 2018 and 2019, respectively. The requested instrumentation provides enhanced sensitivity and capabilities for modern NMR experiments, including temperature-locking, non-uniform sampling, complex waveform generation, magic-angle spinning (MAS) solid-state NMR spectroscopy, and advanced cryogenic probe design for both solution and solid-state MAS applications. The requested instrumentation is essential to the over-riding purpose of the New York Structural Biology Center (NYSBC), a consortium of nine research institutions in New York State, to support the specific aims of the NIH-funded research of the major and minor users of the spectrometer. Research conducted on the 800 MHz NMR Spectrometer ranges from fundamental studies of structure/function of biomolecules, to elucidation of mechanisms of pathogenesis, to discovery of novel lead compounds to pharmaceutical applications. The requested instrumentation will dramatically enhance the capacity of the 800 MHz NMR spectrometer to conduct this research.