IDENTIFICATION OF BIOCHEMICAL MARKERS FOR HUMAN LUNG CANCER

肺癌是所有癌症中导致死亡的主要原因， 是美国癌症死亡率最高的地区 以识别 分子标记，我们使用差异显示方法分析 从两种人肺肿瘤细胞系NCIH 209和NCIH 209中分离的总RNA H69，和细胞系CCD 8Lu，来源于正常人组织。 我们有 鉴定了一个cDNA克隆6-6-1，其在大肠杆菌中以较高水平表达， 小细胞肺癌和非小细胞肺癌 (Non SCLC）细胞与来源于正常肺组织的细胞系的比较。 在相匹配的肿瘤和正常肺组织中，基因6-6-1表达于 肿瘤组织中的水平高于正常组织。 基因6-6-1在大多数人体组织中以低水平表达， 在肾脏、小肠和前列腺中，它的表达水平较高， 程度. 正常人肺组织和正常人肺组织cDNA克隆的DNA序列分析 肾，和肺肿瘤细胞显示，一个小的缺失， 颠换发生在肿瘤细胞中（在一些cDNA克隆中）， 在正常细胞中。 基因6-6-1编码一个相对分子大小的蛋白质 32 kDa。 H69肺癌细胞6-6-1基因突变和缺失 线;两者都导致TAG终止密码子的引入，因此 不能编码32 kDa蛋白质。 全长正常肺的分离 6-6-1基因的cDNA克隆、染色体定位、与 该蛋白的突变、功能及其诊断潜力 肺癌和其他类型癌症的突变正在调查中。

Lung cancer is the leading cause of death among all cancers and it has the highest cancer death rate in the United States. To identify molecular markers, we have used a differential display method to analyze total RNAs isolated from two human lung tumor cell lines, NCI H209 and H69, and a cell line CCD 8Lu, derived from normal human tissue. We have identified a cDNA clone 6-6-1 which is expressed at a higher level in both small cell lung carcinoma (SCLC) and non small cell lung carcinoma (Non SCLC) cells compared to cell lines derived from normal lung tissue. In matched tumor and normal lung tissues, gene 6-6-1 is expressed at higher levels in tumor than in normal tissue. Gene 6-6-1 is expressed at low levels in most human tissues except for kidney, small intestine and prostate, where it is expressed at higher levels. DNA sequence analysis of cDNA clones from normal lung and kidney, and from lung tumor cells revealed that a small deletion and transversion have occurred in tumor cells (in some cDNA clones) but not in normal cells. Gene 6-6-1 encodes a protein of relative molecular size of 32 kDa. 6-6-1 gene is mutated and deleted in H69 lung tumor cell line; both lead to an introduction of TAG termination codon and therefore cannot code for 32 kDa protein. Isolation of full-length normal lung cDNA clone, chromosome localization of 6-6-1 gene, relationship between the mutation and function of the protein and diagnostic potentials of mutations in lung and other types of cancer are under investigation.