IDENTIFICATION OF OXACILLIN RESISTANT STRAINS OF STAPHYLOCOCCUS AUREUS

一项对苯唑西林耐药金黄色葡萄球菌的多中心研究已经到位 应该在一年内完成。噬菌体类型过去是，将来也是 继续用于对苯唑西林耐药菌株进行指纹分析 物种。我们有证据表明，对苯唑西林耐药的菌株 克拉维酸可逆数量很少，苯唑西林 耐药金黄色葡萄球菌在美国许多医院都有一种 优势菌株。苯唑西林耐药性降低的金黄色葡萄球菌 在克拉维酸存在的情况下，高β-内酰胺酶的生产者， 有另一种独特的噬菌体模式。我们还在研究损失率 从处理过的菌株培养出来的苯唑西林耐药性 有耐心的。这一阶段将包括直接进行的初步敏感性测试 从临床样本中提取，然后在连续传代培养过程中进行测试。 这对金黄色葡萄球菌的敏感性检测具有重要意义。 菌株，因为它们开始立即失去抗药性，在最初 文化。 进度： 这项研究已经完成。结果是非常有趣的。它 提供了关于流行性感冒流行病学的确凿数据和新数据 耐苯唑西林金黄色葡萄球菌(ORSA)和产高β-内酰胺酶金黄色葡萄球菌。 金黄色葡萄球菌(HBLPSA)Orsa和HBLPSA是流行性S。 金星。 代表世界范围内第三群噬菌体流行毒株的ORSA是 从临床中心消失。新近分离到的两株ORSA 临床中心，与世界范围内的菌株无关，是社区- 派生的。 HBLPSA是由单一噬菌体组成的V群毒株正在消失 来自临床中心。两株新近临床分离的ORSA 中心和许多美国医院。这种金黄色葡萄球菌菌株具有较高的 导致感染的能力。 苯唑西林耐药损失率测定的研究 体外生长，下降。有些变量被认为太难了 控制，无法获得技术帮助。

A multi-center study of oxacillin resistant S. aureus is in place and should be completed within a year. Phage types have been, and will continue to be used to fingerprint strains of the oxacillin resistant species. We have evidence that strains with oxacillin resistance not reversible by clavulanic acid are few in number, and that oxacillin resistant S. aureus is represented in many U.S. hospitals by a single predominant strain. S. aureus in which oxacillin resistance is reduced in the presence of clavulanic acid, the hyperbetalactamase producers, have another, distinct phage pattern. We also are studying loss rates of oxacillin resistance after the strains are cultured from the treated patient. This phase will include initial sensitivity tests done directly from clinical samples, followed by tests done during serial subcultures. This has important bearing in sensitivity testing of these S. aureus strains, since they begin to lose resistance immediately, on initial cultures. Progress: This study is completed. It turned out to be very interesting. It supplied confirmatory as well as new data on the epidemiology of oxacillin resistant S. aureus (ORSA) and hyperbetalactamase producing S. aureus (HBLPSA). ORSA and HBLPSA are distinct strains of epidemic S. aureus. ORSA representing the world-wide phage group III epidemic strain are disappearing from the Clinical Center. Two recent ORSA isolates in the Clinical Center, unrelated to the world-wide strains, were community- derived. HBLPSA is comprised of a single phage Group V strain are disappearing from the Clinical Center. Two recent ORSA isolates in the clinical Center and in many U.S. Hospitals. This S. aureus strain has an elevated capacity to cause infections. The study to determine loss rates of oxacillin resistance, during in vitro growth, was dropped. Some variables were deemed too difficult to control, and technical help was unavailable.