TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES

逆转录病毒载体已被用于转移和表达cDNA 对于药物代谢酶（细胞色素P450 2A 6和4 B1、FMO和 PHS-1和PHS-2）进入C3 H10 T1/2和AS 52细胞。 c3 h10 t1/2细胞 突变表达人细胞色素P450 2A 6的细胞， 通过烟草特有的亚硝胺NNK进行形态转化。 表达2A 6的AS 52细胞通过NNK突变，突变的2A 6细胞在2A 6细胞中表达。 这种潜在的人类致癌物质的光谱测定。 c3 h10 t1/2细胞 表达细胞色素P450 4 B1的细胞对细胞毒敏感， Ipomeanol和AF的作用，并已被证明是突变的， 异丙肾上腺素 含有兔肺FMO和小鼠PHS-1的AS 52细胞 而PHS-2则是最近推出的。 研究以确定是否 当一种化学物质被不同的P450激活时， 或物种依赖性同种型正在进行中。

Retroviral vectors have been used to transfer and express the cDNAs for drug metabolizing enzymes (cytochromes P450 2A6 and 4B1, FMO, and PHS-1 & PHS-2) into C3H10T1/2 and AS52 cells. C3H10T1/2 cells expressing the human cytochrome P450 2A6 were mutated and morphologically transformed by the tobacco specific nitrosamine, NNK. AS52 cells expressing 2A6 were mutated by NNK and the mutational spectra of this potential human carcinogen determined. C3H10T1/2 cells expressing rabbit cytochrome P450 4B1 were sensitive to the cytotoxic effects of ipomeanol and AF and have been shown to be mutated by ipomeanol. AS52 cells containing the rabbit lung FMO and mouse PHS-1 AND PHS-2 have recently been derived. Studies to determine if mutational spectra vary when a chemical is activated by different P450s or species dependent isoforms are in progress.