SUBCELLULAR COMPOSITION OF THE PANCREATIC ISLETS OF LANGERHANS

我们正在继续进行实验，分析快速冷冻培养的大鼠 在单个分泌颗粒水平上的胰岛。 对薄的干燥冷冻切片的元素分析表明，β（或 B）颗粒可以通过高锌、钙和硫来区分， 而非β（主要是α或A）颗粒含有升高的 磷和镁。 虽然单一的颗粒类型占主导地位 在一个特定的细胞中，在A细胞中发现了一些反叛颗粒， B细胞颗粒的成分指纹 锌，这是发现 在B细胞颗粒中的毫摩尔浓度，被认为是一个标志物 胰岛素储存复合体 数据表明，非B型胰岛 成年胰腺中的细胞可产生含胰岛素的细胞器， 当胰高血糖素和胰岛素共同表达时， 包装在单独的颗粒中。 目前正在努力实现 更好的冷冻条件和冷冻切片质量的改善。

We are continuing experiments to analyze rapidly frozen cultured rat islets of Langerhans at the level of individual secretory granules. Elemental analysis of thin, dried cryosections has shown that beta (or B) granules can be distinguished by high zinc, calcium, and sulfur, whereas non-beta (mainly alpha, or A) granules contain elevated phosphorus and magnesium. Although a single granule type predominated in a particular cell, some rebel granules were found in A cells that had the compositional fingerprint of B-cell granules. Zinc, which was found in millimolar concentrations in B-cell granules, was considered a marker for the insulin-storage complex. The data indicate that non-B islet cells in the adult pancreas may produce insulin-containing organelles and that, when glucagon and insulin are coexpressed, these hormones are packaged in separate granules. Efforts are now in progress to achieve better freezing conditions and improved quality of cryosections.