MOLECULAR CHAPERONES

百日咳杆菌主要分子伴侣蛋白GroEL样蛋白 由该微生物产生的，被提纯。这种蛋白质被发现具有 GroEL家族蛋白质和TO的典型的十四聚体结构 包含与该家族其他成员相似的表位，包括人类 GroEL样蛋白。新生小鼠主动免疫B。 百日咳蛋白对气雾剂攻击几乎没有保护作用 百日咳杆菌。这种蛋白的抗体是由一种 标准白喉-破伤风-百日咳(DTP)疫苗 实验性无细胞百日咳疫苗。因为类似波尔德泰拉·格罗尔的 蛋白质被发现含有与哺乳动物相似的表位。 类似地，存在用标准DTP疫苗接种的可能性 可诱导与哺乳动物GroEL类似物反应的抗体。 旨在确定这种蛋白质可能扮演的角色的研究仍在继续。 在百日咳蛋白的折叠和组装中。

The GroEL-like protein of B. pertussis, the major molecular chaperone produced by this organism, was purified. This protein was found to have the tetradecameric structure typical of the GroEL family of proteins and to contain epitopes similar to other members of this family, including a human GroEL-like protein. Active immunization of neonatal mice with the B. pertussis protein provided little protection against an aerosol challenge with B. pertussis. Antibodies to this protein were elicited in mice by a standard diphtheria-tetanus-pertussis (DTP) vaccine but not by an experimental acellular pertussis vaccine. Since the Bordetella GroEL-like protein was found to contain epitopes similar to those on the mammalian analog, the potential exists that vaccination with standard DTP vaccines may induce antibodies which react with the mammalian GroEL analog. Studies are continuing aimed at identifying the role this protein may play in the folding and assembly of pertussis proteins.