APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY

实验免疫学分院流式细胞仪实验室 支持5名以上研究人员的多个研究项目。这些 研究涉及参数的单细胞定量分析 与从不同物种/组织中新鲜制备的细胞有关，如 以及一系列体外培养的细胞。基础研究支持是 提供给欧洲投资银行的成员以及内部的其他调查人员 DCBDC。目前支持的项目包括但不限于 以下研究领域：a)体内和体外分析 通过T细胞表面分子的细胞内信号传递，b)分析 遗传性或诱导性免疫功能障碍动物的细胞缺陷； C)移植物抗宿主病发病机制研究；d)分析 细胞黏附分子的配位细胞表面表达；e) T细胞个体发育和分化的研究；f)研究 T细胞谱系产生的机制；g)表达分析 移植抗原；h)调查涉及的机制 抗原呈递过程；以及i)机制分析 与骨髓移植排斥反应和接受反应有关。

The Experimental Immunology Branch flow cytometry laboratory currently supports multiple research projects for more than 5 investigators. These investigations involve quantitative single cell analysis of parameters associated with cells freshly prepared from different species/tissues, as well as a spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB as well as to other investigators within DCBDC. Currently supported projects include, but are not limited to, the following areas of study: a) in vivo and in vitro analyses of intra-cellular signalling via T cell surface molecules, b) analyses of cellular defects in animals with genetic or induced immune dysfunction; c) studies of the pathogenesis of graft-versus-host disease; d) analyses of the coordinate cell surface expression of cell adhesion molecules; e) investigations of T cell ontogeny and differentiation; f) studies of mechanisms of T cell repertoire generation; g) analyses of expression of transplantation antigens; h) investigations of mechanisms involved in antigen presentation processes; and i) analyses of the mechanisms involved in marrow graft rejection versus acceptance.