ANTIBODY RESPONSE OF NEONATES TO MATERNAL IMMUNITY, ACTIVE IMMUNIZATION, HORMONES

肺炎链球菌多糖免疫效果的研究 (PS)孕前对新生儿随后抗体反应的影响 PS抗原，雌性小鼠给予从0.5到50的不同剂量 %g或更多的肺炎球菌多糖，例如19A、19F或3PS型， 在交配之前。出生后，将幼鼠分为两组。 其中一组在两周龄时额外给予PS(0.1%g)。 PS处理过的雌性小鼠所生的幼鼠 接受额外剂量的PS的抗体反应比 那些没有接受额外剂量PS的患者。治疗 含500%g的雌性小鼠可引起抗体反应抑制。 在母鼠和幼鼠身上。 来自接受激素治疗的母亲的幼鼠，如生长 激素，或雌激素，然后在怀孕期间接种肺炎球菌疫苗， 对19A PS产生较高的IgM抗体应答。 GRANTZ01BA02020 灭活溶血素(PLY)对小鼠的免疫已被证明 延长强毒肺炎球菌攻击后的存活时间。 因此，灭活PLY可以作为一种有效的蛋白质载体 肺炎球菌多糖(PS)-蛋白结合免疫原。 从大肠杆菌克隆pJCP20中制备灭活的1型肺炎溶血素。 溴化氰与肺炎球菌19F-PS偶联 活化PS与碳二亚胺偶联的方法为偶联。30- 19F PS-蛋白结合物中含有50%的蛋白质。 测定19F PS-PLY结合物的抗体反应。 放射免疫测定法。不同剂量的结合物从0.5到50毫克 在8周龄的小鼠体内注射。5毫克的增强剂注射是 在初诊后7天给药一次，或在初治后7天和14天给药两次 注射。最后一次注射7天后，抗体反应是 量过了。结果表明，该偶联免疫原可诱导 小鼠体内的~(19)F抗体水平显著高于 19F PS。两次加强免疫后小鼠的~(19)F抗体水平 比接受19F的对照组增加了20倍以上 单独使用PS。 肺炎溶血素的抗体应答及对攻击的保护作用 将在接受19F PS-PLY的小鼠身上检测到强毒肺炎球菌 共轭。

To study the effect of immunization with pneumococcal polysaccharide (PS) before pregnancy on the subsequent antibody response of neonates to PS antigens, female mice were given various doses ranging from 0.5 to 50 %g or more of pneumococcal polysaccharides, e.g. type 19A, 19F or 3 PS, before mating. After birth the young mice were divided into two groups. One group was given an additional dose of PS (0.1 %g) at two wks of age. The young mice that were born to PS-treated female mice and that received an additional dose of PS had stronger antibody response than those that did not receive an additional dose of PS. Treatment of female mice with 500 %g induced a suppression of the antibody response in maternal and young mice. Young mice from mothers that received hormone treatment, such as growth hormone, or estrogen followed by pneumococcal vaccine during pregnancy, produced higher IgM antibody responses to 19A PS. GRANTZ01BA02020 Immunization of mice with inactivated pneumolysin (ply) has been shown to increase the survival time after challenge with virulent pneumococci. Thus, inactivated ply can serve as an effective protein carrier for pneumococcal polysaccharide (PS)-protein conjugate immunogens. Inactivated type 1 pneumolysin, prepared from E. coli clone pJCP20 was conjugated to pneumococcal type 19F PS by the cyanogen bromide activation of PS and carbodiimide coupling method for conjugation. 30- 50% protein was contained in the 19F PS-protein conjugate. The antibody response of 19F PS-ply conjugate was measured by radioimmuno-assay. Various doses of conjugate from 0.5 to 50 mg were injected into mice at 8 wks of age. A booster injection of 5 mg was given either once at 7 days, or twice at 7 and 14 days after primary injection. Seven days after last injection the antibody response was measured. The results showed that the conjugate immunogen induced significantly higher 19F Ab levels in the mice than were obtained with 19F PS. The 19F Ab levels in mice that received two booster injections were incresed more than 20 fold over the control group that received 19F PS alone. The antibody response to pneumolysin and protection against challenge of virulent pneumococci will be determined in mice receiving 19F PS-ply conjugate.