PROGRAM IN RETROVIRUSES AND CANCER

这个项目的中心主题是逆转录病毒和 肿瘤改变中的逆转录病毒相关癌基因。这笔赠款包括 七个单独的项目，在广泛的战线上解决这个问题。 项目1关注的是细胞内的位置和功能 由多种病毒癌基因编码的酪氨酸蛋白激酶和 作为这些酶的底物的分子。项目2 利用反义RNA抑制基因表达的尝试 确定此策略是否可用于操纵 C-myc和c-ras癌基因。项目3将探索酪氨酸的作用 多肽激素在分化中作用中的蛋白激酶 和细胞生长。项目4将涉及提纯和微量注射 Myc癌基因的蛋白质分析。项目5涉及 Myc癌基因染色质结构及逆转录病毒的作用 关于myc染色质结构和表达的整合事件。项目6 将研究v-myc基因的特定结构域在 禽髓细胞瘤病毒的致瘤性。Project 7将尝试 确定两个癌基因myc和blym-1在卵巢癌特定阶段的作用 病毒诱导鸡法氏囊淋巴肿大。

The central theme of this program is the role of retroviruses and retrovirus-related oncogenes in neoplastic change. The grant consists of seven individual projects which address the issue across a broad front. Project 1 is concerned with the location and function within the cell of tyrosine protein kinases encoded by a number of viral oncogenes and the molecules which serve as the substrates for these enzymes. Project 2 attempts to exploit the suppression of gene expression by anti-sense RNA to determine whether this strategy can be used to manipulate the expression of c-myc and c-ras oncogenes. Project 3 will explore the role of tyrosine protein kinases in the action of polypeptide hormones on differentiation and cell growth. Project 4 will involve purification and microinjection analysis of the protein of the myc oncogene. Project 5 concerns the chromatin structure of the myc oncogene and effects of retroviral integration events on myc chromatin structure and expression. Project 6 will examine the role of specific domains of the v-myc gene in the oncogenicity of avian myelocytomatosis virus. Project 7 will attempt to determine the role of two oncogenes, myc and Blym-1, in specific stages of virus induced lymphomagenesis in the bursa of Fabricius in chickens.