CONFOCAL MICROSCOPE--3-DIMENSIONAL RECONSTRUCTION

我们申请资金用于共聚焦显微镜， 切片并完成三维重建。 我们将 建立一个联合设施，主要为五个实验室提供服务， 生物系。 拟议的文书将用于 实现NIH资助项目的目标。 五个实验室都是 参与节肢动物神经系统结构和功能的研究 系统. 目前的研究使用分子，遗传，生物化学， 电生理学、行为学和传统解剖学技术。 拟议的仪器将扩大的精度和分辨率 正在进行的工作，并使可能的实验，太耗时 利用现有的工具。 主要的研究工作涉及分子 果蝇发育、记忆、求偶和 昼夜节律 还包括解剖学研究的 神经活性分子如肽类神经递质的分布 果蝇和甲壳类动物的口胃神经系统。 亚细胞 酵母中参与RNA加工的蛋白质的定位， 果蝇的神经元也是预期的。

We request funds for a confocal microscope with the capability to optically section and accomplish three-dimensional reconstructions. We will establish a joint facility that will primarily serve five laboratories in the Biology Department. The proposed instruments will be used to accomplish goals of funded NIH projects. All five laboratories are involved in studies of the structure and function of arthropod nervous systems. Current research uses molecular, genetic, biochemical, electrophysiological, behavioral and conventional anatomical technologies. The proposed instrumentation will extend the precision and resolution of ongoing work, and make possible experiments that are too time-consuming using existing instruments. Major research efforts involve molecular neurogenetic studies of Drosophila development, memory, courtship, and circadian rhythms. Also included are anatomical studies of the distribution of neuroactive molecules such as peptide neurotransmitters in Drosophila and in crustacean stomatogastric nervous systems. Subcellular localizations of proteins involved in RNA processing in yeast and Drosophila neurons are also intended.