TRANSPLANT OF GABAERGIC NEURONS INTO THE SUBSTANTIA NIGRA OF KINDLED RATS

本课题将探讨神经元移植对大鼠脑缺血再灌注损伤的影响 将16日龄大鼠胚胎小脑的悬浮液注入 点燃成年大鼠黑质。 假设是， 黑质参与控制癫痫的蔓延， 将γ-氨基丁酸（GABA）能神经元添加到黑质将 防止癫痫发作 GABA能神经元通过小脑的温和胰蛋白酶消化获得。 16天的胚胎 然后将这些细胞移植到 用哈佛泵在黑质注射 细胞的起源将是 通过用谷氨酸脱羧酶（GAD）抗体染色测定， 以及小脑特异性抗体。 我们最近的研究结果表明，在光显微镜水平上， 移植区域的黑色素细胞。 这些细胞被拉长 形状与广泛的过程。 他们没有沾染上GAD 抗体，但该地区的其他GABA能神经元也没有染色。 我们目前正在调查这背后的原因。 前期工作 已经表明，移植后2周内点燃消失， 回报. 目前正在调查这些调查结果的原因。 的 黑质已被证明是癫痫蔓延的控制点 许多调查员。 目前的研究将试图证明 大鼠胚胎小脑GABA能神经元移植是否 阻止癫痫的蔓延 这些研究的结果大多是负面的。 这些原因 结果可能是多方面的，其中之一是无法种植 GABA能神经元形成突触。 本研究的结论是 大脑移植的神经生物学基础知识 在评估它们在神经系统疾病中的有用性之前，需要进行研究。

This project will investigate the effect of transplanting neuronal suspensions from the cerebellum of 16 day old rat embryos into the substantia nigra of adult kindled rats. The hypothesis is that the substantia nigra is involved in the control of spread of seizures and the addition of gamma amino butyric acid (GABA)ergic neurons to the nigra will prevent the seizures. GABAergic neurons are obtained by gentle trypsinization of the cerebellum of 16 days old embryos. These cells are then transplanted into the substantia nigra using a Harvard pump. The origin of the cells will be determined by staining with the glutamate decarboxylase (GAD) antibody as well as cerebellum specific antibodies. Our most recent results show at a light microscopic level a fine network of cells at the area of transplant in the nigra. These cells are elongated in shape with extensive processes. They have failed to stain with GAD antibody, but other GABAergic neurons in the area have not stained either. We are currently investigating the reasons behind this. Preliminary work has shown that kindling disappears for 2 weeks after transplant but then returns. The reasons for these findings are being investigated. The substantia nigra has been shown to be a controlling point of seizure spread by numerous investigators. The current study will attempt to demonstrate whether transplants of GABAergic neurons from rat embryo cerebellum will block the spread of seizures. The results of these studies were largely negative. The reasons for these findings could be multiple, among them the failure to be able to grow the GABAergic neurons and to form synapses. The conclusions of this study are that further basic knowledge of the neurobiology of brain transplants is needed before their usefulness in neurologic diseases can be evaluated.