APPLICATIONS OF NEW MASS SPECTRAL TECHNIQUES

本项目的目标是研究和开发 新的质谱学技术以提供新的和/或更多 快速解决涉及(1)化学结构的问题 测定、(2)复杂混合物分析和(3)测量 生物系统中的痕量成分。实用程序和作用域 对这些质谱学方法进行了确定，并进行了比较 到其他类型的分析，既有新的，也有已建立的 出去。快原子轰击质谱仪及其组合 液-质联用技术是一种 活期利息。快原子轰击质谱学研究 正离子模式和负离子模式都得到了应用 广泛应用于碱的快速结构测定和 糖基化核苷和合成核苷酸。一个 去除离子污染物的微型海水淡化程序 它们抑制了快原子轰击质量中的样品电离 光谱学已被开发并应用于选定的 人工合成和生物衍生的核苷。关于光谱的研究 FAST过程中样品-基质相互作用产生的异常 原子轰击电离继续进行。

The objective of this project is research on and development of new mass spectral techniques in order provide new and/or more rapid solutions to problems involving (1) chemical structural determination, (2) complex mixture analysis and (3) measurement of trace components in biological systems. The utility and scope of these mass spectral methods are determined, and a comparison to other types of analysis, both new and established, is carried out. Fast atom bombardment mass spectrometry and combined liquid chromatography - mass spectrometry are the techniques of current interest. Fast atom bombardment mass spectrometry in both the positive and negative ion mode has been applied extensively to the rapid structure determination of base and sugar-modified nucleosides and synthetic nucleotides. A microscale desalting procedure to remove ionic contaminants which suppress sample ionization in fast atom bombardment mass spectrometry has been developed and applied to selected synthetic and biologically derived nucleosides. Studies of spectral anomalies generated by sample-matrix interactions during fast atom bombardment ionization continue.