IDENTIFICATION AND ISOLATION OF C-FMS PROTOONCOGEN FROM F344/N RAT LEUKEMIA

原癌基因c-fms在两种成熟的猫科动物中均有发现。 巨噬细胞和未分化的和人肿瘤，以及正常人 骨髓细胞 c-fms原癌基因在 人单核细胞分化和c-fms产物已经被 与单核吞噬细胞生长因子受体相关 （CSF-I）。 本研究的目的是：（1）筛选 c-fms原癌基因在体内连续存在 移植的F344/N单核细胞白血病细胞系，和（2） 启动基因分离和表达的研究 产品 这将提供对肿瘤起源的了解， 有助于提供肿瘤的诊断标准。 此外，本发明还提供了一种方法， 这种肿瘤细胞系可以提供有价值的遗传物质来源， 用于研究c-fms的分子生物学 原癌基因及其对MNCL表达的潜在影响。 我们 研究表明存在分离的c-fms癌基因 来自自发发生的MNCL肿瘤和这些白血病 细胞似乎具有正常的核型（除了不寻常的 亚末端X染色体变异），并具有造血功能 体内单核细胞和淋巴细胞的分化抗原 移植的MNCL肿瘤细胞系。 原位杂交技术使用单层异质 白血病细胞群正在被追踪， c-fms在特定的细胞类型。

The proto-oncogene, c-fms, has been described in both mature cat macrophages and undifferentiated and human tumors, and normal human bone marrow cells. The c-fms proto-oncogene is expressed during human monocyte differentiation and the c-fms product has been related to the receptor for mononuclear phagocyte growth factor (CSF-1). The purpose of this investigation is: (1) to screen for the presence of the c-fms proto-oncogene in the in vivo serially transplanted F344/N mononuclear leukemic cell lines and, (2) to initiate studies on the isolation and expression of the gene product. This will provide insight on the origin of the tumor and aid in providing diagnostic criteria for the tumor. In addition, this tumor line may provide a valuable source of genetic material for investigations into the molecular biology of the c-fms protooncogene and its potential effect on MNCL expression. Our investigation indicates the presence of c-fms oncogene isolated from both spontaneously occurring MNCL tumors and these leukemic cells appear to have a normal karyotype (except for an unusual subterminal x- chromosome variant) and possess hematopoietic differentiation antigens of both monocytes and lymphocytes, in vivo transplanted MNCL tumor lines. In situ hybridization technique using monolayer of heterogeneous leukemic cell population are being pursued to allow identification of c-fms in specific cell types.