NIH/MSU MASS SPECTROMETRY FACILITY

核心研究将继续侧重于执行和 应用集成瞬态记录仪（ITR）， 用于飞行时间（TOF）质量中的时间阵列检测（TIME Array Detection，TOF） 光谱分析，使其有可能检测和整合所有的 从离子加速的离子电流（在整个质量范围内） 这个工具的来源。 预计这一能力将提高 仪器的全光谱灵敏度至少为 数量级，并提高可用的获取率 光谱的数量级。 这两个特性都将具有 对传统GC-MS的深刻影响，特别是随着 重点介绍了毛细管柱在复杂体系分析中的应用 大多数生物样本中的混合物。 现有TOF 仪器已经”用连续源和 电子部分过滤掉具有异常能量的离子。 能源 连续源的滤波和射束脉冲基本上 增加TOF仪器的分辨能力。 对热辅助FAB机理的研究将有助于提高FAB的效率， 继续应用这种新的解吸电离技术 肽、胆汁酸、卟啉等的分析 重要的生物分子。 合作研究将 强调有机代谢分析方法的扩展 尿液中的酸和类固醇，用于研究 代谢在人类疾病状态如糖尿病和糖尿病中的作用。 对外源性物质如污染物和药物的生理反应。 对服务和合作的要求将要求分析 生理液体和其他生化样品中的羧基 酸，糖，吲哚衍生物等。许多项目涉及 有机合成将继续需要精确的测量， 验证其产品的经验公式。 培训将 继续成为基金方案的一个重要特征; 大约20名研究生接受详细的培训， 通过研究项目与五个 专业的研究人员在设施计划。

Core research will continue to focus on the implementation and application of an integrating transient recorder (ITR) which will be used for Time Array Detection (TAD) in time=of-flight (TOF) mass spectrometry to make it possible to detect and integrate all the ion current (over the complete mass range) accelerated from the ion source of this instrument. This capability is expected to increase the full spectrum sensitivity of the instrument by at least an order of magnitude and increase the rate of acquiring useable spectra by an order of magnitude. Both of these features will have a profound effect on conventional GC-MS, especially with increasing emphasis on the use of capillary columns in the analysis of complex mixtures found in most biological samples. An existing TOF instrument has bee" modified with a continuous source and an electric sector to filter out ions with aberrant energy. Energy filtering and beam pulsing of a continuous source substantially increases the resolving power of the TOF instrument. Investigations into the mechanism of thermally assisted FAB will continue as this novel desorption ionization technique is applied to the analysis of peptides, bile acids, porphyrins, and other biologically important molecules. Collaborative research will emphasize extension of metabolic profiling methodology for organic acids and steroids in urine for investigations of altered metabolism in human disease states such as diabetes and of the physiological response to xenobiotics such as pollutants and drugs. Requests for service and collaboration will call for analyses of physiological fluids and other biochemical samples for carboxylic acids, sugars, indole derivatives, etc. Many projects involving organic synthesis will continue to require exact measurements to validate the empirical formulas of their products. Training will continue to be an important feature in the Facility program; approximately 20 graduate students receive detailed training in mass spectrometry through research projects with the five professional investigators in the Facility program.