PHE-MET-ARG-PHE-NH2 LIKE PEPTIDES IN THE BRAIN AND SPINAL CORD

八肽。Phe-Leu-Phe-Gln-Arg-Phe-NH2(F-8-F-NH2)是第一个 用针对软体动物的抗血清检测 心脏兴奋性多肽。Phe-Met-Arg-Phe-NH2，以及随后 从牛脑中分离出来。脑室注射F-8-F-NH2 可降低吗啡的镇痛作用。发现了F-8-F-NH2 高度定位于脊髓背侧胶状质 绳索。在使用体外和体内灌流的研究中 脊髓，我们发现F-8-F-NH2可以通过 氯化钾的去极化浓度(体外)和物质 P，感觉递质(体内)。这一结果进一步支持了 我们的假设F-8-F-NH2可能在 抗伤害感受器。在大鼠体内，F-8-F-NH2也被发现高度 集中在脑垂体腺的后叶。在搜索中 对于这种肽在脑下垂体中的作用，人们发现了F-8-F-NH2 在脑垂体腺中检测不到下限 Brattleboro大鼠，尿崩症大鼠结果表明， Brattleboro大鼠可能是探索其功能的有用模型 脑下垂体F-8-F-NH2。利用免疫组织化学技术，两个 大鼠脑内强F-8-F-NH2免疫反应神经元群 都被揭穿了。其中一处位于脑室周围 下丘脑区和另一个突出的细胞群被发现在 孤束核。这些细胞体是否投射到 目前正在对脑垂体和脊髓进行调查。 本研究的目的是：1)研究 Brattleboro大鼠垂体F-8-F-NH2，2)F-8-F-NH2的释放 脊髓中的NH2及吗啡对其释放的影响 3)F-8-F-NH2免疫反应神经末梢的来源 使用损伤技术的脊髓和脑下垂体。

The octapeptide. Phe-Leu-Phe-Gln-Arg-Phe-NH2 (F-8-F-NH2) was first detected by the antiserum raised against the molluscan cardioexcitatory peptide. Phe-Met-Arg-Phe-NH2, and subsequently isolated from bovine brain. F-8-F-NH2 injected intraventriculary can decrease the analgesic effect of Morphine. F-8-F-NH2 was found to be highly localized in substantia gelatinosa of dorsal spinal cords. In the study using in vitro and in vivo superfusion of spinal cords, we found that F-8-F-NH2 can be released by depolarizing concentration of KCI (in vitro) and also by substance P, the sensory transmitter (in vivo). This result further support our hypothesis that F-8-F-NH2 may have a modulatory role in antinociception. In rats, F-8-F-NH2 was found to be also highly concentrated in posterior lobes of pituitary glands. In searching for the role of this peptide in the pituitary, F-8-F-NH2 was found to be below the limit of detection in pituitary glands of Brattleboro rats, diabetes insipidus rats. The result indicates that Brattleboro rat may be an useful model to explore the function of pituitary F-8-F-NH2. Using Immunohistochemical technique, two strongly F-8-F-NH2 immunoreactive neuronal groups in rat brains were revealed. One of them was located in the periventricular hypothalamic area and another prominent cell group was found in the nucleus tratus solitarii. Whether these cell bodies project to pituitaries and spinal cord are under current investigation. The proposed course of this study is to investigate 1) function of pituitary F-8-F-NH2 using Brattleboro rats, 2) release of F-8-F- NH2 from the spinal cord and the effect of morphine on this release and 3) sources of F-8-F-NH2 immunoreactive nerve terminals in spinal cords and pituitaries using lesion techniques.