LARGE-SCALE PROCESSING OF BIOLOGICAL MATERIALS

试点计划(生物技术)单位结合了几个不同的 活动类型。它负责大规模的 细菌、哺乳动物细胞的生产和生物活性 来自不同来源的化合物。与这项活动平行的是 开展与这些项目相关的流程开发工作 准备工作，以便能够有效地执行这些工作。在……里面 此外，单位并不一定在进行研究工作 与当前项目相关联，但工作时间较长 对单位业绩的影响。 在过去的一年里，该部队开展了150次不同的大型- 规模化制剂，包括微生物从 10到300升，哺乳动物细胞生长到50升体积和 各种生物材料的加工。 在优化大肠杆菌生长方面做了一些开发工作 采用补料分批技术和富氧空气得率100 G/升湿重的生物质。一种新的提纯方法 从铜绿假单胞菌中分离出外毒素A。

The Pilot Plan (Biotechnology) Unit combines several different types of activities. It is responsible for the large-scale production of bacteria, mammalian cells and biologically active compounds from various sources. Parallel to this activity, it is conducting process development work associated with these preparations In order to be able to execute them efficiently. In addition, the unit is carrying on research work not necessarily associated with a current project, but work that has long-term implications for the unit's performance. During the last year, the unit carried out 150 different large- scale preparations, including microorganism growth in volumes from 10 to 300 liters, mammalian cell growth up to 50 liter volumes and processing of various biological materials. Development work was done in the optimization of E. coli growth using the fed batch technique and oxygen-enriched air yielding 100 g/liter wet weight of biomass. A new method for the purification of Exotoxin A from pseudomonas aeruginosa was developed.