ENZYME PURIFICATION--CALMODULIN

钙调蛋白特性研究的有效性取决于 酶制剂纯度的可量化测量和 它所含的钙的比例，并要求提供 极其纯净的试剂、缓冲液和溶剂。分析性 已开发出对象形图进行量化的方法 高纯度钙调素制剂中的钙含量 并在试剂和溶剂中做好准备。 初步分析表明，所需的试剂和溶剂 用于制造超纯酶制剂的人可以自由地 钙等无处不在的污染物的令人满意的水平 使用我们实验室开发的技术。

The validity of studies of calmodulin's properties depends on quantifiable measures of purity of the enzyme preparation and the proportion of calcium it contains, and requires the availability of extremely pure reagents, buffer, and solvents. Analytical methods have been developed for quantitation of picogram quantities of calcium in highly purified preparations of calmodulin and in the reagents and solvents needed to make the preparations. Preliminary analyses show that the reagents and solvents needed for making ultra-pure enzyme preparations can be freed to satisfactory levels from such ubiquitous contaminants as calcium using techniques developed in our laboratory.