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PLASMA MEMBRANE PROTEINS IN THE REGULATION OF CELL BEHAVIOR AND DRUG RESISTANCE

PLASMA MEMBRANE PROTEINS IN THE REGULATION OF CELL BEHAVIOR AND DRUG RESISTANCE
质膜蛋白在细胞行为和耐药性调节中的作用
批准号：
3963038
负责人：
I PASTAN
金额：
$0.0万
依托单位：
DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3963038
关键词：
Golgi apparatus antineoplastic antibiotics cell growth regulation cell membrane colchicine combination chemotherapy complementary DNA diltiazem doxorubicin drug resistance epidermal growth factor gene expression genetic library genetic manipulation ligands lysosomes macroglobulins membrane activity membrane permeability molecular biology molecular cloning mutant natural gene amplification neoplasm /cancer genetics organelles quinidine receptor mediated endocytosis tissue /cell culture verapamil vinblastine

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中文摘要
翻译
对阿霉素（A），长春碱（V）， 秋水仙素（C）和放线菌素（D）（ACD）已被证明含有 导致耐药性的扩增基因（mdr-l）。 的片段 该基因已被克隆并用于筛选cDNA文库和鉴定 编码170 kdalton膜蛋白的cDNA也被发现增加， 多药耐药细胞 170 kd蛋白结合长春碱， 相关药物，这种结合被药物如维拉帕米克服， 奎尼丁和地尔硫卓克服多药耐药性。 水平 在人类癌症中测量MDR-1基因的表达。
英文摘要
Human KB cells which are resistant to adriamycin (A), vinblastine (V), colchicine (C) and actinomycin (D) (AcD) have been shown to contain an amplified gene (mdr-l) responsible for the drug resistance. A fragment of this gene has been cloned and used to screen a cDNA library and identify cDNAs encoding a 170 kdalton membrane protein also found to be increased in multidrug resistant cells. The 170 kd protein binds vinblastine and related drugs, and this binding is overcome by drugs such as verapamil, quinidine, and diltiazem which overcome multidrug resistance. The levels of expression of the mdr-l gene are being measured in human cancers.
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GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
MONOCLONAL ANTIBODIES TO CANCER CELLS
REGULATION OF GENE ACTIVITY
REGULATIION OF CANCER CELL GROWTH AND BEHAVIOR