PROGRAMS FOR COMPUTER-ASSISTED IDENTIFICATION OF BACTERIA

目前商业上不易鉴定的细菌的鉴定 可用的识别工具往往既耗时又困难 任务。在大多数实验室环境中，这些微生物需要有经验的 专家微生物学家或技术专家，确保可靠和准确 身份证明。我们试图让这些身份更多地 通过以下方式使用训练有素的技术人员轻松、准确地获得 为这些目的采用计算机辅助身份识别方法。 现在可以使用以下方法来鉴定四大类细菌 专门开发的计算机程序。这些是革兰氏阳性棒(90 不同物种)；挑剔的革兰氏阴性杆菌(46种不同物种)； 非发酵革兰氏阴性杆菌(不同菌种)与肠杆菌 负棒(81种不同种类)。每个程序都是使用 最少的测试次数和足够的数组用于测试，以允许足够的 每个数据库中的生物体之间的区别。测试也是 根据其易用性和现成可用性进行选择 常规诊断实验室。所有4个计划目前都在进行中 使用过，并且似乎产生了有用的识别结果。建议的 技术人员将标识用作进一步的指导 必要时进行测试，尽管识别通常是明确的 足够了，所以不需要进一步的测试。

Identification of bacteria not readily identified by current commercially available identification kits often is a time-consuming and difficult task. In most laboratory settings, these organisms need an experienced and expert microbiologist or technologist for reliable and accurate identification. We have attempted to make these identifications more readily and accurately available using less well-trained technologists by adapting computer-assisted identification methods for these purposes. Identification of four major groups of bacteria is now possible using specifically-developed computer programs. These are Gram positive rods (90 different species); fastidious Gram negative rods (46 different species); nonfermentative Gram negative rods (64 different species) and enteric Gram negative rods (81 different species). Each program was developed using the minimum number of tests and enough of an array to tests to permit adequate discrimination between the organisms in each data base. Tests were also selected on the basis of their ease of performance and ready availability to routine diagnostic laboratories. All 4 programs are currently being used and seem to yield useful identification results. The suggested identifications are used by the technologists as guidelines for further tests if necessary, although the identifications are usually definitive enough so that further testing is unnecessary.