INFECTION OF HTLV-1-SPECIFIC IMMUNE T-CELL CLONES BY HTLV-I

人嗜T淋巴细胞病毒-I(HTLV-I)特异性T细胞株 建立并克隆了。OKT8+细胞毒克隆(K5)是特异性的 对携带HTLV-I的自体肿瘤细胞有反应，并保留了 正常依赖白介素2(IL-2)。我们观察到多个前病毒 K5细胞中的整合位点(即没有显性前病毒 整合站点)，建议整合站点的数量， 当被占用时，导致T细胞转化是有限的。OKT4+ HTLV-I感染的克隆(R2)对 HTLV-I；但其IL-2非依赖性增殖随时间和时间的延长而增加 这种抗原特异性最终消失了。所有感染HTLV-I的克隆 测试包括另一个OKT8+转化的细胞毒克隆(K7)，该克隆具有 失去对HTLV-I的反应性，表达T细胞受体B链 (TCR-B)信使RNA处于可比水平。两个克隆(K5和K7)具有 TCR-B基因的相同重排，表明它们具有相同的 克隆起源，尽管它们表现出不同的功能特性 在感染HTLV-I之后。 这些数据表明HTLV-I特异性免疫T细胞克隆具有一定的范围 的功能可以感染HTLV-I，保持其免疫反应性 在文化中的不同时期，并失去它，尽管在一些 感染HTLV-I后无功能改变的病例可 检测到。此外，数据表明，不同的后果可能 根据逆转录病毒的模式发生在同一克隆中 感染。

Human T-lymphotropic virus-I (HTLV-I)-specific T-cell lines were established and cloned. An OKT8+ cytotoxic clone (K5) was specifically reactive against HTLV-I-bearing autologous tumor cells, and it retained a normal dependence on interluekin 2 (IL-2). We observed multiple proviral integration sites in K5 cells (i.e., there was no dominant proviral integration site), suggesting that the number of integration sites which, when occupied, result in T-cell transformation is finite. An OKT4+ HTLV-I-infected clone (R2) mounted a specific proliferative response to HTLV-I; however, its IL-2-independent proliferation increased with time and the antigen-specificity was eventually lost. All HTLV-I-infected clones tested including another OKT8+ transformed cytotoxic clone (K7) which had lost its reactivity against HTLV-I, expressed T-cell receptor B chain (TCR-B) messenger RNA at comparable levels. Two clones (K5 and K7) had the same rearrangement of the TCR-B gene, suggesting that they have the same clonal origin, even though they exhibited different functional properties following HTLV-I infection. These data indicate that HTLV-I-specific immune T-cell clones with a range of functions can be infected with HTLV-I, retain their immune reactivity for variable periods of time in culture, and lose it, although in some cases no alteration in function following HTLV-I infection can be detected. In addition, the data suggest that different consequences can take place in the same clone depending on the pattern of retroviral infection.