ORGAN AND SPECIES SPECIFICITIES OF CHEMICAL CARCINOGENS

一种研究人体组织代谢和致突变性的体外方法 化学致癌物的活化已经被开发出来。 刚被隔离， 来自人类和啮齿动物的完整细胞用于代谢， activation. 回复S。鼠伤寒沙门氏菌用于评估生物 代谢物的活性和代谢物的HPLC分析已经被 进行。 过去一年的工作重点是开发一个系统， 用于频繁的人体组织采集。 一个人类系统已经被开发出来 来研究人类个体之间的差异 人类和啮齿类动物肝脏、肾脏和结肠细胞活化之间的关系/ 芳香胺的代谢。 代谢、致突变激活和 个体间的差异已被测量， 在人类个体之间发现的。 这些比较 将人类组织移植到相应的啮齿动物组织的工作正在进行中。

An in vitro approach for studying human tissue metabolism and mutagenic activation of chemical carcinogens have been developed. Freshly isolated, intact cells from humans as well as rodents are used for metabolic activation. Reversion of S. typhimurium is used to assess biological activity of metabolites and HPLC analysis of metabolites have been conducted. Work during the past year has centered on developing a system for frequent human tissue acquisition. A human system has been developed to investigate differences between individual humans and differences between human and rodent liver, kidney and colon cell activation/ metabolism of aromatic amines. Metabolism, mutagenic activation and inter-individual variation has been measured and significant differences found betwen individual humans for these tissues. Comparisons of these human tissues to the respective rodent tissues are in progress.