VIRUSES AND BONE MARROW FAILURE

两种类型的病毒已被检查其在病原学中的作用， 动物和人类的骨髓衰竭。第一种是细小病毒科， 含有动物病毒的最小DNA。 B19人类细小病毒 导致具有潜在红细胞再生障碍的个体中的选择性红细胞再生障碍 溶血 猫泛白细胞减少症病毒是猫疾病的常见病原体， 会导致真正的全血细胞减少和骨髓再生障碍 人类 细小病毒是10年前才发现的， 其研究已经是一个适当的体外细胞培养系统。 使用 红系骨髓细胞的悬浮培养物， 镰状细胞病，我们已经实现了B19的第一次繁殖 剂 该病毒对红细胞系具有高度选择性， 骨髓祖细胞 与复制关联的事件， 这种病毒的转录和蛋白质产生已经在 详细 相反，猫病毒在体外的选择性较低， 对红系和髓系细胞的增殖有较强的抑制作用 细胞 这种病毒也能在猫的悬浮培养物中繁殖 骨髓 对猫逆转录病毒，猫白血病病毒， 表明其对猫细胞体外集落形成的作用是 最小的 在动物研究中，猫白血病病毒对 造血 对人类患者的广泛研究表明， 人类再生障碍性贫血中的逆转录病毒未能显示出 逆转录酶活性或逆转录病毒颗粒通过电子 显微镜

Two types of viruses have been examined for their role in the etiology of bone marow failure in animals and man. The first, the Parvoviridae, are the smallest DNA containing animal viruses. The B19 human parvovirus causes selective erythroid aplasia in individuals with underlying hemolysis. Feline panleukopenia virus is a common agent of cat disease and capable of causing a true pancytopenia and bone marrow aplasia. The human parvovirus was discovered only 10 years ago and a major stumbling block to its investigation has been an adequate in vitro cell culture system. Using suspension cultures of erythroid bone marrow cells from patients with sickle cell disease, we have achieved the first propagation of the B19 agent. This virus is highly selective for erythroid in comparison to myeloid progenitor cells. Events associated with the replication, transcription, and protein production of this virus have been elucidated in detail. The feline virus, in contrast, is less selective in vitro, with a strong inhibitory action on the proliferation of both erythroid and myeloid cells. This virus can also be propagated in suspension cultures of cat bone marrow. Studies of the cat retrovirus, feline leukemia virus, have shown that its effect on in vitro colony formation by cat cells is minimal. In animal studies, feline leukemia virus had marginal effects on hematopoiesis. Extensive studies of human patients of a possible role for a retrovirus in human aplastic anemia had failed to reveal consistent reverse transcriptase activity or retroviral particles by electron microscopy.