Establishment of an MRC Sequencing Hub at the GenePool, the Scottish next-generation genomics facility

阅读我们基因组和其他生物基因组中的DNA代码，彻底改变了医学研究人员的工作。虽然读取基因DNA序列的技术是在40多年前发明的，但直到最近，它在许多研究项目中的使用速度太慢，成本太高。然而，在过去的五年里，新一代的DNA序列阅读器已经发明出来，这些机器的工作速度比旧技术快数千甚至数百万，有望让研究人员在越来越精确、越来越令人兴奋的项目中使用DNA序列。在苏格兰，基因池是爱丁堡大学的一个DNA测序服务，它让整个地区的研究人员从他们的实验中生成DNA序列。基因库已经投资了两台新一代测序仪器，去年读取了超过100亿个碱基的DNA序列（100千兆字节-大约相当于30个人类基因组）。在这项提案中，我们要求英国医学研究理事会提供资金，使我们向苏格兰各地的医学和生物医学研究人员提供DNA序列的能力增加一倍。我们将购买两个以上的当前作物？下一代？我们会继续发展DNA定序仪器，并发展一个高效率及具成本效益的中心，为用户提供DNA数据。我们还将在中期购买第三代DNA序列之一--其输出可能会使新的测序机器相形见绌，就像它们使旧的方法相形见绌一样。我们还将投资于训练有素的实验室技术人员和生物计算科学家，以帮助用户识别其数据中的重要遗传信息。

The reading of the DNA code in our genomes and the genomes of other organisms has revolutionised what can be done by medical researchers. While the technology to read the DNA sequence of genes was invented over 40 years ago, until recently it has been too slow and too expensive to use in many research programmes. However, in the last five years a new generation of DNA sequence readers has been invented, and these machines, working at thousands or millions the pace of the older technology, promise to let researchers use DNA sequence in more and more precise, and more and more exciting, projects.In Scotland, the GenePool is a DNA sequencing service based in the University of Edinburgh that lets researchers throughout the region generate DNA sequences from their experiments. The GenePool has already invested in two of the new generation of sequencing instruments, and read over one hundred thousand million bases of DNA sequence (100 Gigabases - about 30 human genomes-worth) last year. In this proposal we request funding from the UK Medical Research Council to double our ability to deliver DNA sequence to medical and biomedical researchers throughout Scotland. We will purchase two more of the current crop of ?next generation? DNA sequencing instruments, and develop a highly efficient and cost-effective centre to produce DNA data for users. We will also in the medium term buy one of the third generation of DNA sequences - output from which is likely to dwarf the new sequencing machines just as they dwarfed the old methods. We will also invest in highly trained laboratory technologists and biocomputing scientists to help users identify the important genetic information in their data.