MOLECULAR CORRELATES OF NEURONAL FUNCTION

我们提出了建立分子关联的新方法 哺乳动物背根神经节和背根节的功能和结构特征 海马神经元。重点将集中在a)这两个神经元上 使用多个突触介体的人群，以及b)以 哪些关键的分子决定因素是受调节的。具体目标是：1) 测定初级传入神经元和海马神经元的分子特征 区分功能子类和/或形态子类；2) 确定多肽和其他化学介体在细胞内的作用 初级传入神经元和海马神经元的功能；3)检测 膜受体调控的生化机制 渠道，以及这些事件可能与表达 神经元特有的分子决定因素。实验将在#年进行。 在背根神经节和海马神经元的培养中以及在 海马片。已建立的形态(光和电子 显微镜)和电生理(微电极和膜片钳) 这些技术将与免疫细胞化学、生化、 药理学和分子遗传学方法。一种设施，用于 特异性多克隆和单克隆抗体的制备及原位检测 杂交组织化学将由四个人共同使用 项目。

We propose new approaches to establishing molecular correlates of functional and structural features of mammalian dorsal root ganglion and hippocampal neurons. The focus will be upon a) neurons of these two populations that employ multiple synaptic mediators, and b) the manner in which key molecular determinants are modulated. The specific aims are: 1) to determine molecular features of primary afferent and hippocampal neurons that distinguish between functional and/or morphological subclasses; 2) to determine the parts played by peptides and other chemical mediators in the function of primary afferent and hippocampal neurons; 3) to examine the biochemical mechanisms underlying modulation of membrane receptors and channels, and the possible coupling of such events to expression of neuron-specific molecular determinants. The experiments will be done in vivo, in cultures of the dorsal root ganglia and hippocampal neurons and in hippocampal slices. Established morphological (light and electron microscopic) and electrophysiological (microelectrode and patch clamp) techniques are to be coupled with immunocytochemical, biochemical, pharmacological and molecular genetic approaches. A facility for the generation of specific polyclonal and monoclonal antibodies and for in situ hybridization histochemistry will be set up for common use by the four projects.