THREE-DIMENSIONAL HISTOLOGICAL RECONSTRUCTION

一种半自动获取三维结构的系统 有关组织学材料的信息正在开发中。系统 应该比现在有显著的速度和可靠性优势 技术使用连续切片，虽然分辨率可能是有限的。在……里面 简而言之，嵌入的组织块将相对于扫描固定 电子显微镜成像系统，块的表面将被成像 和存储的图像，以及连续的切片将由内置的 切纸机。因此，薄片的处理和配准将 被淘汰了。人类和计算机模式识别将改变 将得到的一组图像转换为三维重建。氧 已经发现，等离子蚀刻可以提供足够的地形浮雕 图像的分辨率现在受到扫描电子显微镜的限制，而不是 制备技术。赫米森达·粗角兽的图像 这项技术与相同组织的电子显微镜图像有很好的相关性， 表明神器的缺乏对于预期的人来说是足够的 学习。

A semi-automatic system for acquiring three-dimensional structural information about histological material is being developed. The system should have significant speed and reliability advantages over present techniques using serial sections, although resolution may be limited. In brief, an embedded tissue block will be fixed relative to a scanning electron microscope imaging system, the surface of the block will be imaged and the image stored, and successive slices will be removed by a built-in microtome. Handling and registration of thin sections will thus be eliminated. Human and computer pattern recognition will transform the resulting set of images into a three-dimensional reconstruction. Oxygen plasma etching has been found to give sufficient topographic relief that the resolution of the images is now limited by the SEM and not by the preparation technique. The images of Hermissenda Crassicornis obtained by this technique correlate well with TEM images of the same tissue, indicating that the lack of artifact is adequate for the contemplated studies.