MECHANISM OF CELLULAR INFILTRATION OF THE EYE IN ANTERIOR UVEITIS

Lewis大鼠皮下注射细菌内毒素的实验研究 会在24小时内产生急性眼内炎 句号。这种内毒素诱导的葡萄膜炎(EIU)的特征是 双相蛋白渗出和由以下组成的细胞渗出 巨噬细胞和中性粒细胞(PMN)。我们用了这个 外周细胞浸润机制的模型研究 器官。通过注射脂多糖(沙门氏菌)诱导EIU 小鼠)350fg/kg。细胞因子诱导的中性粒细胞水平 每隔两小时测定一次血清和血清中的趋化物质(CINC) 在眼睛的房水里。中国石油天然气集团公司的生产动力学 体循环迅速上升，两小时后达到峰值 LPS注射，随后在以下方面逐渐下降 八个小时。在眼睛中，CINC水平高于血清水平 EIU诱导后10小时的水平，对应于 细胞渗入。56对白细胞浸润的抑制作用 注射抗黏附分子Ab1B6(2 mg/只)的百分率为 仅伴有轻微的房水减少 分别为9%和16%的水平，表明 眼内CINC由眼细胞产生。的具体效果 当直接眼内注射CINC时，眼内CINC被证实 250 ng纯化的CINC之后是显著的PMN 渗出，在没有蛋白质渗出的情况下。数据 证明由眼部产生的趋化因子 组织参与外周血肿的炎症反应 注射脂多糖。

The subcutaneous injection of bacterial endotoxin in Lewis rats produces an acute intraocular inflammation evolving over a 24-hour period. This endotoxin-induced uveitis (EIU) is characterized by a biphasic protein exudation and a cellular infiltrate composed of macrophages and polymorphonuclear neutrophils (PMN). We used this model to study the mechanism of cellular infiltration in peripheral organs. EIU was induced with an injection of LPS (Salmonella typhimurium) at 350 fg/kg. The level of cytokine-induced neutrophil chemoattractant (CINC) was measured every two hours in the serum and in the aqueous humor of the eye. The kinetics of CINC production in the systemic circulation showed a rapid rise, peaking two hours after LPS injection, followed by a progressive decline over the following eight hours. In the eye, the CINC levels increased above the serum levels 10 hours after EIU induction, corresponding to the time of cellular infiltration. The inhibition of leukocyte infiltration by 56 and 64 percent with the antiadhesion molecule Ab 1B6 (2mg/rat) was accompanied by only a slight reduction in the aqueous humor CINC levels of nine and 16 percent respectively, indicating that the intraocular CINC was produced by ocular cells. The specific effect of CINC in the eye was confirmed when a direct intraocular injection of 250 ng of purified CINC was followed by a significant PMN infiltration, in the absence of protein exudation. The data demonstrate that the production of chemotactic factors by ocular tissue participates in the inflammatory reaction induced by peripheral LPS injection.