COLLABORATIVE PROJECTS IN ENVIRONMENTAL HEALTH SCIENCES

该项目包括那些质谱学 工作组与其他小组在内部和外部进行协作 解决共同关心的问题的机构。的主要关注点 这些项目是：1)未知化合物的结构测定；2) 识别和/或确认生物途径；3) 量化；以及4)为结构制定战略 生物重要化合物的测定。当前的协作 正在进行的项目包括：1)确定孤儿的配体 受体(C.Weinberger，LRDT)；2)花生四烯酸定量 代谢物(D.Zeldin，LPP)和3)质谱学鉴定 自旋俘获自由基(R.Mason，LMB)。项目说明 下面展示了各种各样的项目，其中质谱学 能够起到不可或缺的作用。孤儿受体--我们支持Dr。 Weinberger的研究发现了孤儿的原生配基 受体，其生理作用尚不清楚。现在 该项目是维甲酸X受体RXR的鉴定。 花生四烯酸代谢物的定量--最灵敏和 生理样品中二十烷类化合物的准确定量方法 通过负离子化学物质下的选择离子监测(SIM) 电离(NICI)质谱仪。我们目前正在对环氧树脂进行量化- 和二羟基二十碳三烯酸。环氧二十烷类化合物的量化 是一项研究的一部分，该研究首次确定了这些化合物的形成 人体心脏中的时间及其调控蛋白的鉴定 队形。自旋捕获自由基的鉴定--我们的主要工作 在这一领域的努力已经得到了网上的开发和应用 LC/EPR/MS功能。我们是第一组(仍然是仅有的 两组)，以成功地结合这些技术。我们目前在中国的工作 这一区域是细胞色素c和细胞色素c的自旋加合物的鉴定。 肌红蛋白。这些血红素蛋白可能形成氧化自由基。 与衰老、癌症和心肌缺血有关。

This project includes those projects in which the mass spectrometry workgroup collaborates with other groups, within and without the Institute to solve problems of mutual interest. The major focuses of these projects are: 1) structure determination of unknown compounds; 2) identification and/or confirmation of biological pathways; 3) quantitation; and 4) development of strategies for the structure determination of biologically important compounds. Current collaborative projects under way include; 1) identification of ligands for orphan receptors (C. Weinberger, LRDT); 2) quantitation of arachidonic acid metabolites (D. Zeldin, LPP), and 3) mass spectrometric identification of spin-trapped free radicals (R. Mason, LMB). The project descriptions below demonstrate the wide variety of projects in which mass spectrometry can play an integral role. Orphan Receptors - We are supporting Dr. Weinberger's research into identifying native ligands to 'orphan receptors', receptors whose physiological role is unclear. The present project is the identification of retinoic acid X receptor, RXR. Quantitation of Arachidonic Acid Metabolites - The most sensitive and accurate method for quantitation of eicosanoids in physiological samples is by selected ion monitoring (SIM) under negative ion chemical ionization(NICI) mass spectrometry. We are currently quantifying epoxy- and dihydroxy-eicosatrienoic acids. The quanitation of epoxy eicosanoids is part of a study identifying formation of these compounds for the first time in human heart and identification of the protein regulating that formation. Identification of Spin-Trapped Free Radicals - Our major effort in this area has been the development and application of on-line LC/EPR/MS capabilities. We were the first group (and still one of only two groups) to successfully couple these techniques. Our current work in this area is the identification of spin adducts of cytochrome c and myoglobin. Oxidation radical formation of these hemeproteins maybe implicated on on aging, cancer and myocardial ischemia.