FACTORS INFLUENCING GENETIC TRANSCRIPTION--INITIATION AND TERMINATION

核糖核酸酶H(RNase H)在人乳头瘤病毒复制中起关键作用 逆转录病毒，包括人类免疫缺陷病毒(艾滋病毒) 参与正常的细胞活动，包括DNA复制。这个 这个项目的目的是为了详细了解 核糖核酸酶H底物的精确性质，即 细胞核糖核酸酶H来源于细胞来源的酶具有更高的 比那些逆转录病毒来源的特异性活性更高，并有一定的 不同的特点。我们发现核糖核酸酶H的分离 MuLV逆转录酶的结构域来自聚合酶结构域 一种不同于亲本核糖核酸酶H的活性酶 但仍具有较低的比活性。如果我们生产核糖核酸酶H 酵母，它们有很高的比活性，无论它们的 与自己的特殊域名联系在一起。这些结果表明， 这些蛋白质的差异在于RNaseH结构域本身。

Ribonuclease H (RNase H) plays crucial roles in replication of retroviruses, including the human immunodeficiency virus (HIV) as well as involvement in normal cellular events including DNA replication. The purpose of this project is to gain a detailed understanding of the precise nature of the substrates for RNases H, the chemical makeup of cellular RNases H. The enzymes derived from cellular sources have higher specific activities than those of retroviral origin and have somewhat different specificities. We have found that separation of the RNase H domain of MuLV reverse transcriptase from the polymerase domain creates an active enzyme with specificities different from the parental RNase H but still with low specific activity. If we produce RNases H from yeasts, they have high specific activities regardless of their association with their own special domains. These results indicate that the differences in these proteins reside in the RNase H domain itself.