SPECTROSCOPY FOR ALZHEIMER'S DISEASE DIAGNOSIS

描述（来自申请人摘要的逐字记录）：提出的研究 这里将导致一个实用的，非侵入性的，比较 利用近红外诊断阿尔茨海默病（AD）的廉价方法 (NIR)低能量激光光谱学AD是光谱分析的理想候选者。 诊断.独特的生化标志物（老年性）的存在 含有淀粉样纤维和神经纤维缠结的斑块 磷酸化的蛋白质）将提供独特的光谱特征。 研究人员开发脑氧监测仪并研究脑光学 断层扫描显示脑组织对NIR是高度半透明的 波长，并且足够的光可以被传输和检测通过 一个完整的成人头骨来做诊断最高的区域 AD标志物的集中点是颞叶，位于太阳穴下方 头骨最薄的地方使用的激光能量是安全的， 产生和检测这些波长所需的波长是容易获得的。的 器械预期在床边使用，以测量 疾病，一个基本的能力，以确定疗效的建议 治疗和监测疾病进展。该提案代表了 麻省理工学院激光生物医学研究中心（LBRC）， 在光谱诊断研究和贝德福德VA医疗中心（VAMC）， AD研究的领导者LBRC成功证明了癌症的诊断 在使用激光诱导荧光的各种器官（口腔，结肠，膀胱， 乳腺）和冠状动脉定量生化分类 堵塞物和血液分析物（葡萄糖、白蛋白），使用拉曼散射。 在LBRC进行的比较正常和AD标本的初步研究 颞皮质和颞骨，由VAMC提供（包括显微镜下 和大量样品），在荧光和荧光光谱中显示出非常有希望的特征， 拉曼光谱。这项工作将确定适当的签名选择， 设计临床系统所需的波长和算法。特别是 体外前瞻性研究表明，近红外荧光光谱法 检测人脑组织中的AD。这项工作将建立适当的 设计临床治疗所需的特征、波长和算法的选择 系统

DESCRIPTION (Verbatim from the Applicant's Abstract): The research proposed here will lead to the development of a practical, non-invasive, comparatively inexpensive method for diagnosing Alzheimer's disease (AD) using near-infrared (NIR) low energy laser spectroscopy. AD is an ideal candidate for spectroscopic diagnosis. The demonstrated presence of unique biochemical markers (senile plaques containing amyloid fibrils and neurofibrillary tangles rich in phosphorylated proteins), will provide unique spectroscopic signatures. Researchers developing cerebral oxygen monitors and studying brain optical tomography have shown that brain tissue is highly translucent to NIR wavelengths and that sufficient light can be transmitted and detected through an intact adult skull for diagnostic purposes. The region of highest concentration of AD markers is the temporal lobe, located beneath the temple where the skull is thinnest. The laser energy used is safe, and the components needed to generate and detect these wavelengths are readily available. The device is intended for use at the bedside in order to measure the degree of disease, an essential capability for determining the efficacy of proposed curative treatments and monitoring disease progress. This proposal represents a collaboration between the MIT Laser Biomedical Research Center (LBRC), a leader in spectroscopic diagnostic research and the Bedford VA Medical Center (VAMC), a leader in AD research. LBRC has successfully demonstrated diagnosis of cancer in various organs using laser induced fluorescence (mouth, colon, bladder, breast), and quantitative biochemical classification of coronary artery blockages and blood analytes (glucose, albumin), using Raman scattering. Preliminary studies performed at the LBRC comparing specimens of normal and AD temporal cortex and temporal bone, provided by VAMC (including both microscopic and bulk samples), show very promising signatures in both fluorescence and Raman spectra. This work will establish the appropriate choices of signatures, wavelengths and algorithms needed to design a clinical system. In particular, a prospective study in vitro demonstrated near-infrared fluorescence spectroscopy detects AD in human brain tissue. This work will establish the appropriate choices of signatures, wavelengths and algorithms needed to design a clinical system.