SELECTION OF AEDES AEGYPTI REFRACTORY TO DENGUE VIRUS

黄热病蚊子，埃及伊蚊，是一种众所周知的传播媒介， 黄热病（YF）和登革热（DEN）黄病毒。 亚种 和Ae的人口。埃及人的传播能力差异很大， 黄病毒 研究表明中肠的感染屏障 存在于西非亚种Ae中。台湾埃及 在 其他亚种，埃及伊蚊，我们证明，病毒 复制受控制播散性感染的基因调控 率 在这两个亚种中，病毒感染的水平在数量上有所不同 在个体之间，并受到随机环境影响。 最近的分子和统计学进展使遗传学家能够绘制基因座 影响数量性状的表达。 这些被称为 数量性状基因座（QTL）。 这个项目的第一个目标是绘制 控制埃及伊蚊中DEN病毒载体感受态的QTL。 本项目产生的遗传标记将作为 埃及伊蚊群体中DEN易感性的生物标志物 墨西哥登革热流行区。 这是另一个特定的目标 由巴里比蒂博士提交的提案，以回应本PA。 所提出的工作的第二个目标是使用标记辅助 选择（MAS）以产生携带中肠的埃及伊蚊菌株 屏障基因与调节播散性 感染率 这些菌株可能对DEN完全不敏感 病毒，并将提供一个特殊的机会，以阐明 DEN传播的遗传机制。 这些菌株可 也有可能释放到登革病毒流行地区 传输 Beaty提案的另一个具体目标是， 墨西哥DEN流行地区的遗传隔离人群。

The yellow fever mosquito, Aedes aegypti, is a well known vector of yellow fever (YF) and dengue fever (DEN) flaviviruses. Subspecies and populations of Ae. aegypti vary widely in their ability to transmit flaviviruses. Studies have shown that a midgut barrier to infection exists in the West African subspecies Ae. aegypti formosus. In the other subspecies, Ae aegypti aegypti, we demonstate that viral replication is regulated by genes that control disseminated infection rate. In both subspecies, levels of virus infection vary quantitatively among individuals and are subject to random environmental effects. Recent molecular and statistical advances enable geneticists to map loci affecting the expression of quantitative traits. These have been termed quantitative trait loci (QTLs). The first goal of this project is to map QTLs that control vector competence for DEN virus in Ae aegypti. The genetic markers arising from this project will be tested as biomarkers of DEN susceptibility in populations of Ae aegypti in dengue endemic areas in Mexico. This is a specific aim of another proposal being submitted by Dr. Barry Beaty in response to this PA. The second goal of the proposed work is to use marker-assisted selection (MAS) to generate strains of Ae aegypti that carry the midgut barrier genes in combination with genes that regulate the disseminated infection rate. These strains may be completely refractory to DEN virus and will provide an exceptional opportunity to elucidate the genetic mechanisms underlying DEN transmission. These strains may also have the potential for release into endemic areas of DEN virus transmission. Another specific aim of the Beaty proposal is to identify genetically isolated populations in DEN endemic regions of Mexico.