A cell atlas of the human outflow tract of the heart

影响心脏流出道(OFT)的缺陷占所有先天性心脏病病例的三分之一。它们通常会导致新生儿血液循环和/或氧合异常，出生后不久就需要手术矫正。OFT的正确发展和功能依赖于不同细胞类型之间的相互作用。这些类型的细胞含有相同的遗传指令，但由于它们表达不同的基因，因此会产生不同的蛋白质。我们将使用新技术，这将使我们能够表征单细胞表达的基因，将人类OFT组织解构为其组成细胞类型，并追踪胚胎发育期间细胞的不同来源。总而言之，这些结果将为如何建立健康和患病的细胞状态提供新的见解，并在疾病诊断和个性化和再生医学中产生连锁影响。

Defects affecting the outflow tract (OFT) of the heart represent a third of all congenital heart disease cases. They often result in abnormal blood circulation and/or oxygenation in the neonate and require surgical correction shortly after birth. Correct development and function of the OFT depend on the interaction between different cell types. These cell types contain the same genetic instructions but produce different proteins because they express different genes. We will use new technologies, which will allow us to characterize single-cell expressed genes, to deconstruct the human OFT tissue into its constituent cell types and trace the diverse origins of the cells during embryonic development. Collectively, these results will provide new insight into how healthy and diseased cell states are established, with knock-on implications in disease diagnosis and personalized and regenerative medicine.