Engineering Development of Laser Capture Microdissection

激光捕获显微切割是一种技术 由美国国立卫生研究院开发，用于从 用于后续分子分析的组织病理切片。一个 与组织样品接触的热塑性薄膜选择性地 由激光在微观控制下激活，以与 想要的组织。去除薄膜会将粘连的组织分离 从幻灯片上剩余的部分中提取出来，以便进行分析。 该技术使收集和处理极其重要的 均质样品以常规方式进行，不需要 非常灵巧的手工操作。在过去的几年里，一些 这项技术的改进已经开发出来了。一个圆柱形的 使用与该组织的“线”接触的构造已经被 完美的，最大限度地减少污染，并能够 去除直径小至3微米的组织碎片。 使用连续热塑性塑料胶带的几个系统 正在开发中。已经开发了一个热模型来 描述激光能量被吸收的过程以及 在聚合物中扩散。这使得能够预测组织和 薄膜温度瞬变是系统参数的函数。

Laser Capture Microdissection is a technique developed at NIH to capture minute portions of tissue from histo-pathology slides for subsequent molecular analysis. A thermoplastic film in contact with a tissue specimen is selectively activated by a laser beam under microscopic control to bond with the desired tissue. Removal of the film separates the adhered tissue from that remaining on the slide, making it available for analysis. The technique enables the collection and processing of extremely homogeneous samples in a routine manner that does not require great manual dexterity. Over the past several years a number of refinements of the technique have been developed. A cylindrical configuration employing a "line" contact with the tissue has been perfected which minimizes contamination, and is capable of removing tissue fragments as small as 3 microns in diameter. Several systems utilizing a continuous tape of thermoplastic are under development. A thermal model has been developed to describe the process by which the laser energy is absorbed and diffused in the polymer. This enables the prediction of tissue and film temperature transients as a function of the system parameters.