CORE--SCIENTIFIC

科学核心的目的是使各种 转基因和基因敲除突变体，原代细胞培养，一般 分子生物学技术、生化分析和形态学 并对四个项目进行了组织学方法的研究。这个 这些服务的提供将使调查人员能够进行 他们的研究与各种质量控制方法的一致性 以一种对单个项目来说不可能的方式进行分析 来实现。这个科学核心的一个特别的优点是，所有的 项目负责人正在积极参与执行 每月进行实验，并向他人提供自己的专业知识 研究研讨会、会议。例如，陈博士(核心领导)将 提供他在使用超氧化物歧化酶转基因和 基因敲除突变小鼠与氧自由基相关的生物化学 和分子生物学。夏普博士将提供他在基因方面的专业知识 表达(原位杂交和免疫细胞化学)，DG 脑放射自显影和[~(14)C]安替比林放射自显影术 血液流动。诺布尔博士将提供她在形态学方面的专业知识 对神经元损伤的评估(包括光和超微结构)。Dr。 Swanson将提供他在.oh自由基的高效液相色谱测量方面的专业知识 和H_2O_2及脑梗塞体积的成像定量测量 分析。此外，该项目的协调人Sylvia Chen博士 Science Core，将提供她在分子分析方面的专业知识 基因表达(聚合酶链式反应、逆转录-聚合酶链式反应)、原代细胞培养和各种 生化分析。因此，这个科学核心提供了一个独特的 各种项目实现其研究目标的工具 统一的方法和专业知识相辅相成。

The purpose of the Scientific Core is to make available various transgenic and knockout mutants, primary cell cultures, general molecular biological techniques, biochemical assays, and morphological and histological methods to the four projects of the program. The availability of these services will allow investigators to conduct their studies with consistency in quality control of various methods and assays in a way that would not be possible for individual projects to achieve. One particular strength of this Scientific Core is that all the project leaders are actively participating in the execution of the experiments and provide their own expertise to others during monthly research seminars meetings. For example, Dr. Chan (Core Leader) will provide his expertise in the use of superoxide dismutase transgenic and knockout mutant mice as well as oxygen radicals-related biochemistry and molecular biology. Dr. Sharp will provide his expertise in gene expression (in situ hybridization and immunocytochemistry), DG autoradiography and [14C iodoantipyrine autoradiography for cerebral blood flow. Dr. Noble will offer her expertise in morphological assessments (both light and ultrastructure) of neuronal injury. Dr. Swanson will offer his expertise in HPLC measurements of .OH radicals and H2O2 and quantitative measurements of infarct volume using imaging analysis. In addition, Dr. Sylvia Chen, the coordinator of the Scientific Core, will provide her expertise in molecular analysis of gene expression (PCR, RT-PCR), primary cell culture and various biochemical assays. Thus, this Scientific Core provides an unique vehicle for various projects to achieve their research goals with a unified methodology and expertise that will complement one another.