DELAYED EXTRACTION UV & INFRARED WAVELENGTH MALDI MS

我们设计并建造了一个坚固而廉价的高 用于进行延迟提取MALDI的电压脉冲系统 飞行时间MS测量。 我们用这个系统来运行 我们的线性飞行时间仪器， 连续提取（CE）或延迟提取（DE）模式。 使用CE和DE的UV-和IR-MALDI的比较导致了一些 有趣的发现，我们将在一份手稿中报告， 准备. 因此，例如，我们已经发现IR-MALDI产生 明显比UV-MALDI更冷的蛋白质离子， IR-和UV-MALDI光谱都源自于 钠离子与蛋白质。

We have designed and constructed a robust and inexpensive high voltage pulsing system for carrying out delayed-extraction MALDI time-of-flight MS measurements. We have used this system to run both of our linear time-of-flight instruments either in continuous-extraction (CE) or delayed-extraction (DE) modes. Comparison of UV- and IR-MALDI using CE and DE has led to a number of interesting discoveries which we will report in a manuscript in preparation. Thus, for example, we have found that IR-MALDI produces demonstrably cooler protein ions than UV-MALDI and that the tails on both IR- and UV-MALDI spectra originate from multiple association of Na cations with the protein.