PHOSPHORYLATION SITES IN PEPTIDES W/ MULTIPLE PUTATIVE PHOSPHORYLATION SITES

蛋白质梯状测序是我们在2005年开发的一种技术。 实验室对多肽进行测序，包括那些具有修饰的 个氨基酸 其中一个重要的潜在应用是 这项技术是研究蛋白质磷酸化。 了解 不同蛋白激酶的特异性，并进一步探索 梯形测序的潜力，一个模型肽，其中包含 多个丝氨酸磷酸化位点，化学合成。 这种肽被用于研究几种 不同的蛋白激酶和磷酸化的化学计量。 此外，不同磷酸化（位点和数目）的模型肽 (same序列如上述肽）将被合成以检查 我们的质谱技术是否可以用来获得 关于磷酸化的化学计量学的详细信息。 目前正在编写一份介绍这些结果的文件。

Protein ladder sequencing is a technique developed in our laboratory to sequence polypeptides including those with modified amino acids. One of the important potential applications of this technique is the study of protein phosphorylation. To understand the specificities of different protein kinases and to further explore the potentials of ladder sequencing, a model peptide, which contains multiple serine-phosphorylation sites, was synthesized chemically. This peptide is being used to study the specificities of several different protein kinases and the stochiometries of phosphorylation. In addition, variously phosphorylated (site and number) model peptides (same sequence as above peptide) will be synthesized to examine whether our mass spectrometric technique can be used to obtain detailed information concerning the stochiometry of phosphorylation. A paper describing these results is in preparation.