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DEVELOPING AN ANIMAL MODEL FOR ATOPIC DERMATITIS

DEVELOPING AN ANIMAL MODEL FOR ATOPIC DERMATITIS
开发特应性皮炎动物模型
批准号：
6265238
负责人：
FU-TONG LIU
金额：
$2.74万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-12-01 至 1999-11-30
来源：
https://reporter.nih.gov/project-details/6265238
关键词：
SCID mouse atopic dermatitis clinical research disease /disorder model helper T lymphocyte human subject model design /development

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中文摘要
翻译
本研究的目的是建立一种特应性皮炎的动物模型，因为目前还没有针对这种情况的好的动物模型。它包括从特应性皮炎患者中分离外周血单个核细胞(PBMC)。PBMC(特别是CD4+T细胞)已被证明与这种疾病的发病机制有关。一旦从特应性供者中分离出PBMC，它们就被植入SCID小鼠体内。在PBMC植入后，抗原被引入小鼠皮肤，试图触发人类CD4+细胞渗透到皮肤中。然后获取皮肤活检进行组织学分析，以及各种细胞因子的分析(通过RNA检测)，以查看特应性皮炎表型是否已经出现。
英文摘要
This study is aimed at developing an animal model for atopic dermatitis, since no good animal model for this condition yet exists. It involvesisolating peripheral blood mononuclear cells (PBMC's) from atopic dermatitispatients. PBMC's (specifically CD4+ T cells) have been shown to be involved in the pathogenesis of this disorder. Once the PBMC's have been isolated from the atopic donors, they are engrafted into SCID mice. After the PBMC's have engrafted, antigens are introduced to the murine skin, in an attempt to trigger human CD4+ cell infiltration into the skin. Skin biopsies are then obtained for histological analysis, along with analysis for various cytokines (by RNA detection) to see if the atopic dermatitis phenotype has arisen.
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