CRYSTAL STRUCTURE OF HUMAN RHOA GDP RHOGDI COMPLEX & ITS BIOLOGICAL IMPLICATIONS

从酵母中选择的蛋白质代表了进化上的多样性 家庭成员不被预测对应于已知的 蛋白质结构（基于Sali Lab酵母蛋白质模型）。 的 初始蛋白质家族选自在所述细胞上产生的簇。 序列相似性的基础上，由MANUMERS，PRODOM，和DOMO。 编码 通过PCR从基因组DNA获得的序列被克隆，表达， 纯化并结晶用于结构测定。 信息 关于蛋白质提供和进展的测定 结构总结在数据表中。 蛋白质P013-P016 被选中来测试结构预测工具是否真的 发现蛋白质之间的结构相似性， 氨基酸序列的一致性为14 - 22%。 主要目的是 建立所需的技术，以增加对 蛋白质折叠的多样性。 这些基本信息是 需要开发计算工具来预测蛋白质 从基因序列和预测蛋白质功能 从结构。 这些工具最终将被应用于理解 这些蛋白质的功能和相互作用 人类蛋白质组 有关该计划的资料，请浏览 http://genome5.bio.bnl.gov/Proteome/

Proteins from yeast are chosen to represent evolutionarily diverse families whose members are not predicted to correspond to a known protein structure (based on Sali Lab Yeast Protein Models). The initial protein families were selected from clusters generated on the basis of sequence similarity by SYSTERS, PRODOM, and DOMO. Coding sequences obtained by PCR from genomic DNA are cloned, expressed, purified and crystallized for structure determination. Information about the proteins is provided and progress toward determination of the structures is summarized in the data tables. Proteins P013-P016 were chosen to test whether the structure prediction tools did in fact discover structural similarity between proteins that align with only 14-22% identity in amino-acid sequence. The primary objective is to put in place the technology needed to increase knowledge of the diversity of protein folds in nature. This basic information is needed to develop computational tools for predicting protein structures from gene sequences and for predicting protein function from structure. These tools will ultimately be applied to understand the functions and interactions of the 100,000 or so proteins of the human proteome. Information on the project is available at http://genome5.bio.bnl.gov/Proteome/