FOLDING OF INFLUENZA VIRUS GENES

来自经γ辐照的D2 XRII鼠的细胞的共培养 骨髓基质细胞系 白细胞介素-3/粒细胞-巨噬细胞集落刺激因子 （GM-CSF）依赖性造血祖细胞系FDC-P1 JL 26 刺激因子非依赖性造血细胞的出现 子线 有证据表明M-CSF或一种蛋白质 与D2 XRII细胞表达的M-CSF（LSF）抗遗传相关，可 都对独立因素的出现起到了作用 子线 为了分离出一种与 从D2 XRII cDNA文库中分离M-CSF分子克隆， 与小鼠M-CSF基因探针杂交 其中两个分子 克隆，命名为60.8.2和6452。 含有885个碱基对（bp） M-CSF编码区缺失。 这样的cDNA克隆尚未被发现。 以前在小鼠中描述过，但与之同源的cDNA克隆 已从人胰腺肿瘤细胞系MIA-PaCa-2中分离。 检测到三个转录本（4.8kb、3.4kb和1.8kb）， 与RNA转录物特异性的寡核苷酸杂交 删除60.8.2。 这些被删除的记录并不 似乎是由电离辐射诱导的;然而，另外两个M-CSF 大小为4.4kb和2.3kb的转录物显示增加的积累 在伽马辐照之后。 逆转录-聚合酶链 RT-PCR结果显示，缺失特异性转录本（8） 不仅在D2 XRII细胞中，而且在几种小鼠骨中也检测到 骨髓基质细胞系和几种小鼠组织。

Cocultivation of cells from the gamma irradiated D2XRII murine bone marrow stromal cell line with Interlcukin-3/granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent hematopoietic progenitor cell line FDC-PlJL26 stimulates the emergence of factor independent hematopoietic cell sublines. Several lines of evidence suggested that M-CSF or a protein antigenetically related to M-CSF (LSF) expressed by D2XRII cells, may have played a role in the emergence of the factor independent sublines. In an effort to isolate a factor antigenetically related to M-CSF, molecular clones were isolated from a D2XRII cDNA library that hybridized to a mouse M-CSF, genetic probe. Two of these molecular clones, designated 60.8.2 and 6452. contained an 885 base-pari (bp) deletion in the M-CSF coding region. Such a cDNA clone has not been previously described in the mouse but a cDNA clone homologous to it has been isolated from a human pancreatic tumor cell line, MIA-PaCa-2. Three tra nscripts (4.8 Kb, 3.4 kb, and 1.8kb) were detected that hybridized to an oligonucleotide that was specific to RNA transcripts containing the 60.8.2 deletion. These deleted transcripts do not appear to be induced by ionizing irradiation; however, two other M-CSF transcripts of sizes to 4.4 kb and 2.3 kb show increased accumulation after gamma irradiation. Reverse transcription-polymerase chain reaction (RT-PCR) revealed that the deletion specific transcript (8) were detected not only in D2XRII cells but also in several mouse bone marrow stromal cell lines and in several mouse tissues.