FOLDING OF INFLUENZA VIRUS GENES

野生型A/AA/6/60原始分离株的全部8个基因 流感病毒和冷适应的A/AA/6/60疫苗病毒 进行了克隆、测序和RNA折叠分析。两者都有 对雪貂来说，病毒是t和弱毒的。为了完成这项研究，一个 野生型的同一种病毒，既不是ts，又是强毒，将是 测序并进行RNA折叠分析。的关联性 RNA对温度敏感和寒冷的表型具有折叠性 适应以及vRNA的核外运输将是 探索过了。这一系列研究的第二个目标是 将计算机生成的RNA折叠预测与实验进行比较 演绎结构。SJCRH拥有包含92个非结构基因的数据库 序列(流感病毒最小的基因--890个碱基)。我 建议使用Zuker算法研究他们预测的RNA折叠 Y-MP来确定NS基因的折叠是否会落入 对应于来源主机的组以及是否保守的域 胞核脱核等功能所必需的可能是 已确认身份。然后我会将电脑预测的折叠与 用绿豆核酸酶和绿豆核酸酶实验推断结构 补骨脂素交联剂的研究。

All eight genes of the original isolate of wild type A/AA/6/60 influenza virus and of the cold adapted A/AA/6/60 vaccine virus have been cloned, sequenced and subjected to RNAfold analysis. Both viruses are ts and attenuated for ferrets. To complete the study, a wild type of the same virus which is both non-ts and virulent will be sequenced and subjected to RNAfold analysis. Relevance of the RNAfolds to the phenotypes of temperature sensitivity and cold adaption as well as to transport of vRNA out of the nucleus will be explored. The second objective of this series of studies is to compare computer generated RNAfold predictions to experimentally deduced structures. SJCRH has a database of 92 nonstructural gene sequences (the smallest gene - 890 bases - of the influenza virus). I propose to study their predicted RNAfolds using the Zuker algorithm on the Y-MP to determine whether the folds of the NS gene will fall into groups corresponding to host of origin and whether conserved domains necessary for functions such as exodus from the cell nucleus may be identified. I will then compare the computer predicted folds to experimentally deduced structures using mung bean nuclease and psoralen cross-linking studies.