A versatile mass spectrometry platform for quantitative proteomics underpinning biomedical research at The University of Sheffield.

蛋白质和代谢物最能代表细胞或有机体在某一特定时刻的状态。因此，这些蛋白质和代谢物（蛋白质组和代谢组）的总收集是生物学或个人健康的非常有用的读数。分析蛋白质和代谢物是具有挑战性的，因为有许多不同的类型和不同的特征。然而，一种叫做质谱法的分析方法非常适合于鉴定和重要的是，大量这些生物分子的定量。我们建议在我们的质谱研究设施中安装一个多功能质谱平台，为整个大学的研究人员提供最先进的蛋白质组和代谢组分析。该仪器将使我们能够增加我们可以分析和比较的样品数量，对现有技术具有挑战性的样品进行更详细的分析，并允许在一系列样品中精确测量特定的蛋白质和代谢物。谢菲尔德大学的研究人员将利用这项技术来了解抗菌素耐药性、神经变性和癌症背后的分子过程，并识别患者样本中的分子变化，以开发和测试治疗一系列疾病的新药。

Proteins and metabolites best represent the state of a cell or organism at a given moment in time. The total collection of these proteins and metabolites (proteomes and metabolomes) is, therefore, a very informative readout of biology or an individual's health. Analysing proteins and metabolites is challenging due to the many different types and diverse characteristics. However, an analytical approach called mass spectrometry is well suited to both the identification and, importantly, quantification of a vast range of these biological molecules. We propose installing a versatile mass spectrometry platform in our mass spectrometry research facility to provide state-of-the-art proteome and metabolome analysis for researchers across the university. This instrument will allow us to increase the number of samples we can analyse and compare, perform a more detailed analysis of samples that are challenging with existing technology and permit precise measurement of specific sets of proteins and metabolites in a range of samples.Researchers at The University of Sheffield will use this technology to understand the molecular processes underlying antimicrobial resistance, neurodegeneration and cancer and to identify molecular changes in patient samples to develop and test novel drugs to treat a range of diseases.