CALCIUM SENSITIVE ADENYLYL CYCLASES

描述(摘自申请者摘要)：分子和细胞生物学 这些方法将被用来研究钙敏感的腺苷酸的机制 环化酶(Acs)定位于高钙结构域。使用等离子体进行的实验 膜分离程序，胆固醇消耗和 将进行免疫沉淀以解决细胞基础 急性冠脉综合征与多种钙通道的共定位。HIGH的作用机制 将首先解决AC5和AC6的钙亲和力抑制问题 缺失突变。钙对低亲和力抑制的机制及其作用 对MG监管的可能依赖将通过现场定向来解决 在一些不同调控的ACS的C1a区域发生突变， 开发关于腺酰环化酶晶体结构的发展信息。

DESCRIPTION (Adapted from Applicant's Abstract): Molecular and cell biological approaches will be used to examine the mechanisms whereby Ca-sensitive adenylyl cyclases (ACs) are targeted to domains of high Ca. Experiments using plasma membrane fractionation procedures, cholesterol depletion and immunoprecipitation will be conducted to address the cellular basis for colocalization of ACs and a variety of Ca-entry channels. The mechanism of high affinity inhibition by Ca of AC5 and AC6 will be addressed initially be deletion mutagenesis. The mechanisms of low afffinity inhibition by Ca and its possible dependence on regulation by Mg will be addressed by site-directed mutagenesis in the C1a region of a number of differently-regulated ACs, exploiting developing information on the crystal structure of adenylyl cyclase.