KINETIC STUDIES OF INDIUM LABELED LEUKOCYTES

新鲜、冻融或培养的人白细胞的动力学模式 通过用111 -铟（一种放射性同位素）标记离体细胞进行研究， 同位素标记，并测量其在整个身体的分布， 伽马照相机成像和血样的伽马计数的手段。这 这类研究有着广泛的应用。最常见的用法 放射性标记的白细胞运输研究是脓肿定位， 疑似感染病例未得到其他非传染性疾病确诊， 侵入性研究。在这些情况下，自体或同种异体粒细胞， 通过简单的静脉切开术或通过单采血液成分术收集，用50 μ Ci标记 11 l-铟/kg患者体重。14篇研究报告 在临床中心患者中进行了脓肿定位 在过去的6年里参加了各种方案。其中两个14 结果呈阳性，并对一例沙门氏菌进行了适当的治疗 骨髓炎在血友病患者与艾滋病毒感染，并切除 左-埃二氏综合征患者憩室脓肿。在 许多阴性研究，不必要的手术探查， 避免了 铟标记的白细胞运输研究也进行了调查 作为诊断工具， 高安氏动脉炎合作进行的前瞻性研究 NIAID和NMD/CC显示，放射性标记的自体单核细胞 细胞运输不能准确预测是否有症状的患者 发烧和高沉降率有活动性动脉炎，因此 不能用来确定是否干预抗炎 代理商必须启动。 与NCI外科分支的合作试验已经调查了 放射性标记的自体骨髓移植的诊断效用和预后应用 转移性肝癌患者的肿瘤浸润淋巴细胞（TIL）研究 黑素瘤TIL运输研究显示， 临床上未检测到，并且TIL向肿瘤部位的运输是强烈的。 与TIL细胞输注后肿瘤消退相关， 环磷酰胺 最近，放射性标记的同种异体粒细胞运输研究 已被用于确定是否肺浸润患者 儿童慢性肉芽肿病（COD）是由于感染或 肺瘢痕和肉芽肿的形成。COD中的贩运研究 具有HLA同种免疫的患者已被用于预测是否 同种异体细胞在输血后仍能存活， 粒细胞输注的治疗试验是必要的。

The kinetic patterns of fresh, frozen-thawed, or cultured human leukocytes are studied by tagging the cells ex vivo with 111 - Indium, a radio- isotopic label, and measuring their distribution throughout the body by means of gamma camera imaging and gamma counting of blood samples. This type of study has widespread applications. The most common use of these radio-labeled leukocyte trafficking studies is for abscess localization in cases of suspected infection not definitively diagnosed by other, non- invasive, studies. In these cases autologous or allogeneic granulocytes, collected by simple phlebotomy or by apheresis, are labeled with 50 muCi of 11 l-Indium per kg of patient weight. Fourteen studies for the purpose of abscess localization were performed in Clinical Center patients enrolled in a variety of protocols in the past 6 years. Two of these 14 were positive and led to appropriate therapy for a case of Salmonella osteomyelitis in a hemophiliac patient with HIV infection, and resection of a diverticular abscess in a patient with Zollinger-Ellison syndrome. In many of the negative studies, unnecessary surgical exploration was avoided. Indium-labeled leukocyte trafficking studies have also been investigated as diagnostic tools is-detecting large vessel inflammation in patients with Takayasu's arteritis. A prospective study performed in collaboration with NIAID and NMD/CC showed that radio-labeled autologous mononuclear cell trafficking could not accurately predict whether symptomatic patients with fever and high sedimentation rates had active arteritis, and thus could not be used to determine whether intervention with anti-inflammatory agents should be initiated. Collaborative trials with the Surgery Branch, NCI, have investigated the diagnostic utility and prognostic application of radio-labeled autologous tumor-infiltrating lymphocyte (TIL) studies in patients with metastatic melanoma. TIL trafficking studies revealed metastatic deposits that were undetected clinically, and TIL trafficking to sites of tumor was strongly correlated with tumor regression in response to infusions of TIL cells and cyclophosphamide. Most recently, radio-labeled allogeneic granulocyte trafficking studies have been used to determine whether pulmonary infiltrates in patients with chronic granulomatous disease of childhood (COD) are due to infection or to lung scarring and granuloma formation. Trafficking studies in COD patients with HLA-alloimmunization have been used to predict whether allogeneic cells will survive following transfusion and thus whether a therapeutic trial of granulocyte transfusions is warranted.