RESTRICTION FRAGMENT LENGTH POLYMORPHISMS IN NORMAL AND NEOPLASTIC TISSUES

研究项目的主要目标是确定和描述 与肾癌和小细胞癌发病有关的基因 肺癌。我们今年取得的主要成就是： 1.另一种胚系错义VHL突变(547T)的鉴定 至C)与常见的无肾细胞的嗜铬细胞瘤有关 癌症。 2.证明VHL基因突变产生某些特定的 在西方VHL家族中的肿瘤产生相同的肿瘤谱 日本的VHL家庭。 3.Cos-7细胞中VHL蛋白pVHL/vhlin的检测 将VHL基因导入小鼠体内。流行性出血热病毒蛋白的检测 肾癌细胞的细胞核。 4.开发一种显示生长的生物检测方法 VHL基因对肾癌细胞的抑制作用。 5.一个加拿大乳头状肾大家族的鉴定 细胞癌。

The major goal of the research project is to identify and characterize genes involved in the pathogenesis of kidney cancer and small cell carcinoma of the lung. Our major accomplishments this year are: 1.The identification of another germline missense VHL mutation (547 T to C) associated with frequent pheochromocytomas without renal cell carcinoma. 2.The demonstration that VHL gene mutations that produce certain tumors in Western VHL families produced the same tumor spectrum in Japanese VHL families. 3.The detection of the VHL protein, pVHL/vhilin, in Cos 7 cells transfected with the VHL cDNA. The detection of the VHL protein in the nucleus of renal carcinoma cells. 4.The development of a biological assay that demonstrates the growth suppressive effects of the VHL gene in renal carcinoma cells. 5.The identification of a large Canadian family with papillary renal cell carcinoma.