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MOUSE MODEL--TARGETED GENE KNOCK OUT OF CYTOSOLIC PHOSPHOLIPASE A2

MOUSE MODEL--TARGETED GENE KNOCK OUT OF CYTOSOLIC PHOSPHOLIPASE A2
小鼠模型——胞质磷脂酶 A2 的靶向基因敲除
批准号：
6162135
负责人：
R LANGENBACH
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/6162135
关键词：
active sites carcinogen testing carcinogens complementary DNA cytoplasm disease /disorder model electroporation gene mutation gene targeting genetic recombination genetically modified animals inflammation laboratory mouse neoplasm /cancer nucleic acid sequence phospholipase A2

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中文摘要
翻译
工作总结：通过以下方法破坏编码cPLA2的小鼠基因： 同源重组目前正在进行中。缺陷的小鼠 功能性cPLA2将通过靶向该基因的区域而产生 编码丝氨酸228处的酶的活性位点，已知丝氨酸228是 对酶活性至关重要。 一种靶向结构正在 电穿孔到胚胎干细胞中，并将靶细胞注射到 2.5日龄小鼠胚泡以产生嵌合体。缺陷的小鼠 cPLA2将用于确定该酶在癌症中的功能， 炎症
英文摘要
Summary of Work: The disruption of the mouse gene encoding cPLA2 by homologous recombination is currently in progress. Mice deficient in functional cPLA2 will be generated by targeting the region of the gene encoding the active site of the enzyme at serine 228 which is known to be essential for enzymatic activity. A targeting construct is being electroporated into embryonic stem cells and targeted cells injected into 2.5 day old mouse blastocysts to produce chimeras. Mice deficient in cPLA2 will be used to determine the function of the enzyme in cancer and inflammation.
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会议论文
MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
COMPARATIVE GENETIC TOXICOLOGY OF H.C. BLUE 1 AND H.C. BLUE 2
TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
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