SOLID STATE MR OF BONE MINERAL

描述（改编自申请人的摘要）：本发明的中心思想 项目是固态磁共振（SSMR）成像， 光谱学可以为与光谱学相关的问题提供独特的解决方案。 生物矿化化学在这种发展的延续中， 用于骨矿物质表征的SSMR方法，体内和高 分辨率SSMR技术应用于研究重塑 合成磷酸钙生物材料在体内，并阐明 骨矿物晶体的结构化学性质。工作重点 用现有技术无法进行的测量。 在具体目标1中，将非专利β-三钙 磷酸盐（TCP）植入到兔骨缺损模型的宿主骨中 随后进行体内和高分辨率SSMR。虽然许多努力 一直在研究这种和类似的越来越多地使用钙 磷酸盐基材料，这些材料很少被研究， 固态MR，并且从未通过该方法在体内。45只兔子 以及植入物重塑的时间过程 材料将通过体内SSMR成像和高场 高分辨交叉极化/魔角旋转（CP/MAS） 光谱学、化学分析和组织形态计量学 标本这项研究预计将阐明的化学细节， 到目前为止仍然不清楚的重塑过程。具体目标 2、专门的CP/MAS等固态MR技术的结合 与计算机建模应用于表征骨矿物晶体 为了开发结构化学的详细模型， 水晶内部以及表面。松质骨标本来自 正常和卵巢切除的大鼠，用阿仑膦酸盐处理和未处理， 一种典型的二膦酸盐抗再吸收药物， 在骨质疏松症的治疗中越来越重要，将进行分析。 因为二膦酸盐的作用包括物理化学效应 包括与骨矿物质晶体的结合，增强了对 骨矿物晶体及其二膦酸盐结构化学 结合性能在设计改进的抗- 吸收性药物

DESCRIPTION (Adapted from Applicant's abstract): The central idea of this project is that solid state magnetic resonance (SSMR) imaging and spectroscopy can provide unique solutions to problems related to the chemistry of biomineralization. In this continuation of the development of SSMR methodology for bone mineral characterization, in vivo and high resolution SSMR techniques are applied to study the remodeling of synthetic calcium phosphate biomaterials in vivo, and to elucidate the structural chemical properties of bone mineral crystals. The work focused on measurements that can be made in no other way with existing technology. In Specific Aim 1, the conversion of a non-proprietary beta-tricalcium phosphate (TCP) implant into the host bone in a rabbit bone defect model is follow with in vivo and high resolution SSMR. Although much effort has been expended in the study of this and similar increasingly used calcium phosphate based materials, these materials have rarely been studied by solid state MR, and never in vivo by this method. Forty-five rabbits will be implanted, and the time course of the remodeling of the implant material will be characterized by in vivo SSMR imaging, and by high field high resolution cross polarization/magic angle spinning (CP/MAS) spectroscopy, chemical analysis and histomorphometry on harvested specimens. This study is expected to elucidate details of the chemistry of the remodeling process that have so far remained obscure. In Specific Aim 2, specialized CP/MAS and other solid state MR techniques in conjunction with computer modeling are applied to characterize bone mineral crystals in order to develop detailed model of the structural chemistry of the crystal interior as well as the surface. Specimens of trabecular bone from normal and ovariectomized rats, treated and not treated with alendronate, a typical diphosphonate anti-resorptive pharmaceutical of rapidly increasing importance in the treatment of osteoporosis, will be analyzed. Because the action of diphosphonates includes physicochemical effects involving binding to bone mineral crystals, enhanced understanding of the structural chemistry of bone mineral crystals and their diphosphonate binding properties will be important in the design of improved anti- resorptive drugs.