BRAINSTEM RESPIRATORY NEURON INTERACTIONS

这个项目的目标是定义脑干网络机制。 通过它高碳酸血症，颈动脉化学感受器，颈动脉 压力感受器和皮肤伤害性感受器塑造呼吸运动 图案。多阵列记录技术，脉冲序列分析，以及 尖峰触发的小区内记录的平均值将用于 测量连接的许多神经元的反应和功能联系 同时在延髓中缝核、桥脑呼吸群、 和Bot-zinger腹侧呼吸组(BOT-VRG)。 对人工通风的猫进行去大脑处理。派生自 这些数据将通过计算机模拟进行测试。计划中的实验 将回答五个假设。 假设1：桥脑和延髓网络共同作用于 调节呼吸运动模式以响应：a)脑干 高碳酸血症(即中枢化学感受)；b)颈动脉化学感受器 刺激，c)颈动脉压力感受器刺激，d)皮肤 伤害性感受器刺激。 假设2：多个反射系统共享脑桥延髓网络 呼吸运动模式的调节。 假设3：桥脑延髓网络包括内部检查和 调节颈动脉持续时间和大小的平衡 化学感受器和压力感受器反射对呼吸的作用。 假设4：脑桥延髓网络中的压力感受器反射 调节呼吸上的伤害性反射反应。 假设5：反射性诱导的瞬时和长期构型 脑桥延髓网络产生的时空模式 同步性，在传统的射速测量中并不明显， 对机器人采取行动-VRG。 改善呼吸系统和呼吸系统疾病的治疗和管理 心血管控制依赖于对神经的更好理解 管理这些功能的系统。这个项目将是对 目前在体内和体外与其他组织的研究进展 简化论方法，并确定以下网络站点和流程 遗传性或获得性异常可能会扰乱呼吸。

The goal of this project is to define brain stem network mechanisms through which hypercapnia, carotid chemoreceptors, carotid baroreceptors, and cutaneous nociceptors shape the respiratory motor pattern. Multi-array recording technology, spike train analysis, and spike-triggered averaging of intracellular recordings will be used to measure the responses and functional associations of many neurons corded simultaneously in the medullary raphe nuclei, pontine respiratory group, and Botzinger-ventral respiratory group (BOT-VRG) of anesthetized or decerebrate artificially ventilated cats. Network models derived from these data will be tested with computer simulations. Planned experiments will address five hypotheses. Hypothesis 1; Pontine and medullary networks operate together to modulate the respiratory motor pattern in response to: a) brain stem hypercapnia (i.e., central chemoreception); b) carotid chemoreceptor stimulation, c) carotid baroreceptor stimulation, and d) cutaneous nociceptor stimulation. Hypothesis 2: Multiple reflex systems share pontomedullary networks in the regulation of the respiratory motor pattern. Hypothesis 3: Pontomedullary networks include internal checks and balances that regulate the duration and magnitude of carotid chemoreceptor and baroreceptor reflex actions on breathing. Hypothesis 4: Baroreceptor reflex actions in pontomedullary networks modulate nociceptive reflex actions on breathing. Hypothesis 5: Reflexively induced transient and long-term configurations of pontomedullary networks generate spatiotemporal patterns of synchrony, not apparent in conventional measures of firing rate, that act on the BOT-VRG. Improved treatments and management of disorders of respiratory and cardiovascular control depend on improved understanding of the neural system that regulates these functions. This project will complement advances currently being made with other in vivo and in vitro reductionistic approaches and identify network sites and processes where inherited or acquired abnormalities could disrupt breathing.