CRYSTALLOGRAPHIC STUDIES OF INTERLEUKIN 5 RECEPTOR

我们已经结晶了β亚基的胞外区。 白介素5受体。这是一种重要的细胞因子受体 它与哮喘和某些疾病的发病机制有关 寄生虫感染。晶体的衍射力很弱，它可以 在旋转的阳极上收集完整的数据集最多需要10天 消息来源。晶体的有效分辨率受 数据采集系统的信噪比为-3.5A 旋转的阳极和同步加速器上的-2.6A。为了方便 经过衍生化反应，已经产生了许多半胱氨酸突变体。 一些突变的晶体只长到很小的尺寸。这些不是 当使用旋转阳极源时会产生衍射光。事实证明， 这些晶体在14 BM-C上产生了有用的衍射。

We have crystallised the extracellular domain of the beta subunit of the interleukin-5 receptor. This is an important cytokine receptor that has been implicated in the pathogensis of asthma and certain parasite infections. The crystals are weakly diffracting and it can take up to 10 days to collect a complete dataset on a rotating anode source. The effective resolution of the crystals is limited by the signal to noise ratio of the data collection system, being -3.5A on a rotaing anode and -2.6A on a synchrotron. In order to facilitate derivativisation, a number of cysteine mutants have been produced. Some of the mutant crystals grow only to a small size. These do not diffract when a rotating anode source is used. It has been shown that these crystals produce usable diffraction on 14 BM-C.