STRUCTURAL STUDIES OF THE RNA COMPONENT OF RNASE P

说明(摘自摘要)：这是一份修订后的提案。RNA是中心 与许多重要的细胞过程有关，这在核中也是独一无二的 酸能够催化化学反应。尽管它很重要， 人们对大于50的RNA分子的原子结构知之甚少 核苷酸。申请者对大RNA的结构感兴趣 分子，因此将注意力集中在核糖核酸酶的RNA成分上 P(核糖核酸酶P)。核糖核酸酶P是负责切割5‘端的内切酶 导致tRNA成熟的前-tRNA。来自中国的核糖核酸酶P RNA(P RNA) 枯草芽孢杆菌和大肠杆菌由两个独立的结构域组成。 结构域I长约150个核苷酸(约52 kDa)，涉及 在tRNA识别中。申请者已经明确了来自两个B的域I。 枯草杆菌和大肠杆菌，并已开始阐明它们的原子结构。 这个项目的长期目标是了解它的结构和功能 核糖核酸酶P-RNA。这一提议的具体目的是：阐明原子 枯草杆菌P-RNA结构域I的研究。水晶已经在手中，而且 它们的衍射率至少为4.0A，以改善B的结构域I的晶体。 枯草杆菌P-RNA能够获得原子分辨率信息。为了澄清 大肠杆菌P RNA(M1RNA)结构域I的结构。初步结晶有 已经被获取了。比较枯草杆菌结构域I的结构 和E.Coli，以确定对它们的相似性和 不同之处。 关于P-RNA的工作与其他关于大RNA分子的研究有关，在 特别是对催化RNA分子的研究。

DESCRIPTION (Adapted from abstract): This is a revised proposal. RNA is central to a multitude of important cellular processes and is also unique among nucleic acids in being able to catalyze chemical reactions. Despite its importance, very little is known about the atomic structure of RNA molecules larger than 50 nucleotides. The applicants are interested in the structure of large RNA molecules and hence have focused attention on the RNA component of ribonuclease P (Rnase P). RNase P is the endonuclease responsible for cleavage of the 5' end of pre-tRNA leading to tRNA maturation. Both the Rnase P RNA (P RNA) from Bacillus subtilis and Escherichia coli are formed by two independent domains. Domain I is approximately 150 nucleotides long (about 52 kDa) and is involved in tRNA recognition. The applicants have crystallized domain I from both B. subtilis and E. coli and have started to elucidate their atomic structures. The long term goal of this project is to understand the structure and function of RNase P RNA. The specific aims of this proposal are: To elucidate the atomic structure of domain I of B. subtilis P RNA. Crystals are already in hand, and they diffract to at least 4.0 A. To improve the crystals of domain I of B. subtilis P RNA to be able to obtain atomic resolution information. To elucidate the structure of domain I of E. coli P RNA (M1 RNA). Preliminary crystals have already been obtained. To compare the structure of domain I from B. subtilis and E. coli to identify the features important for their similarities and differences. The work on P RNA is relevant to other studies of large RNA molecules and, in particular, to the study of catalytic RNA molecules.