CORE--LABORATORY

作为他们工作的自然进展，该计划项目的研究人员Grant将利用转基因或基因中断(敲除)小鼠来确定肾脏内特定基因过度表达或缺乏表达的影响。核心设施(核心B)将向不同项目的首席调查人员提供转基因和基因敲除小鼠。核心还将准备Promoter-Reporter结构，并提供不同项目的调查人员。核心实验室还将准备Promoter-Reporter结构，并提供肾脏组织的组织学分析，包括形态、巨噬细胞渗透、核因子-kappaB亚型、平滑肌肌动蛋白表达等。核心实验室(核心B)将供参与该计划项目赠款的所有研究人员使用。

As a natural progression of their work investigators of the Program project Grant will utilize transgenic or gene disrupted (knockout) mice to determine the impact of over expression or lack of expression of specific genes within the kidney. The core facility (Core B) will provide transgenic and knockout mice to the Principal Investigators of the different projects. The core will also prepare Promoter-Reporter constructs and provide Investigators of the different projects. The core will also prepare Promoter-Reporter constructs and provide histological analyses of kidney tissue, including morphology, macrophage infiltration, NF-kappaB isotypes, smooth muscle actin expression etc. The core laboratory (Core B) will be utilized by all of the investigator participating in this Program Project Grant.