BIOCONTAINED HIGH SPEED CELL SORTER

该申请是部分资金，用于购买匹兹堡大学癌症研究所（UPCI）流式细胞术共享资源设施内的生物含高速荧光激活细胞分选仪。该核心设施的任务是为生物医学领域的所有研究小组提供细胞悬浮液的高速无菌分选，包括那些正在开发细胞治疗临床前试点研究的研究小组和那些需要分选潜在生物危害材料的研究小组。通过Cellomation和Forma Scientific之间的合作安排，我们制定了MoFlo/R高速分选机生物包容安装的规范，其中所有射流都将被隔离在生物安全外壳中。MoFlo/R血细胞计数器的模块化设计可以追溯到劳伦斯利弗莫尔国家实验室的格尔货车登恩博士设计的原型。采用模块化设计的主要原因之一是便于定制安装，如此处所提出的。货车登恩博士已同意担任这一独特的安装顾问。所要求的资金仅用于仪器。如果获得这笔赠款，UPCI已同意支付安装费用，包括购买和定制改造密封装置的费用。高速细胞分选技术最近已经得到了改进，新一代仪器可以以比我们现有设备快5至10倍的速度常规处理细胞。因此，PHS资助的现在依赖于细胞分选的研究可以以更具成本效益的方式进行，并且细胞分选可以扩展到那些现在依赖于其他效率较低的细胞分离方法的项目。据我们所知，在生物环境中的拟议定制安装是独一无二的，将为这种昂贵的仪器提供显著的附加值。

This request is for partial funds to purchase a Biocontained High-Speed Fluorescence Activated Cell Sorter within the University of Pittsburgh Cancer Institute (UPCI) Flow Cytometry Shared Resource Facility. The mandate of this core facility is to provide access to high-speed aseptic sorting of cell suspensions for all research groups within the biomedical area, including those who are developing pre-clinical pilot studies for cellular therapy and those with the need to sort potentially biohazardous materials. Through a collaborative arrangement between Cytomation and Forma Scientific, we have developed specifications for a biocontained installation of the MoFlo/R high-speed sorter in which all fluidics will be isolated in biosafety enclosure. The modular design of the MoFlo/R cytometer dates to the prototype designed by Dr. Ger van den Engh at the Lawrence Livermore National Laboratory. One of the major reasons for the modular design was to facilitate custom installations such as that proposed here. Dr. van den Engh has agreed to serve as a consultant for this unique installation. The funds requested cover instrumentation only. Should this grant be awarded, the UPCI has agreed to cover the costs of installation including purchase and custom modification of the containment device. Technology in high speed cell sorting has recently improved to the extent that the new generation of instruments can routinely process cells at rates 5- to 10-fold faster than is possible with our existing equipment. Thus PHS funded research which now relies on cell sorting can be carried out in a more cost-effective fashion,a nd cell sorting can be extended to those projects which now rely on other less effective methods of cell separation. The proposed custom installation in a biocontained environment is, to our knowledge, unique and will provide significant added value to this costly instrumentation.